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Merck
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主要文件

LH20100

Supelco

Sephadex® LH-20 Size Exclusion Resin

140-550 mesh

别名:

亲脂交联葡聚糖®

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About This Item

UNSPSC代码:
13111000
NACRES:
NB.21
价格与库存信息目前不能提供

产品名称

Sephadex® LH-20,

表单

slurry

技术

LPLC: suitable

基质

Agarose

基质活性基团

polymer

溶胀

1 g swells to 4 mL (water, methanol)

珠子尺寸

25-100 μm

工作pH范围

2-13

分离技术

size exclusion (SEC)

储存温度

room temp

相关类别

应用

Sephadex® Lh-20是一种交联葡聚糖基树脂，可结合有机溶剂流动相用于凝胶渗透、正相分配，以及脂质、类固醇、脂肪酸、激素，维生素等的吸附色谱层析。此外，这一产品还用于凝胶过滤色谱、蛋白质色谱，凝胶过滤介质、树脂和分离介质。
Sephadex® Lh-20已用于从黑莓基因型中分离生物活性多酚成分[1]，以及从莲藕根茎水性提取物中分离色氨酸。[2]

法律信息

Sephadex is a registered trademark of Cytiva

替代产品

产品编号
说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000426250
0000426250
0000426005
0000426005
0000409842

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Min Xu et al.
Biology of reproduction, 81(3), 587-594 (2009-05-29)
In vitro ovarian follicle cultures may provide fertility-preserving options to women facing premature infertility due to cancer therapies. An encapsulated three-dimensional (3-D) culture system utilizing biomaterials to maintain cell-cell communication and support follicle development to produce a mature oocyte has
Y Jiang et al.
International journal of food sciences and nutrition, 61(4), 346-356 (2010-05-15)
Various vegetables were investigated for antioxidant activities in two assays, namely, inhibition of lysis of erythrocytes induced by peroxyl radicals and inhibition of lipid peroxidation. The lotus (Nelumbo nucifera Gaertn) rhizome showed the strongest antioxidant activity in both assays. The
The source, composition and flux of polycyclic aromatic hydrocarbons in sediments of the river Derwent, Derbyshire, U.K.
Evans, K.M., et al.
Water, Air, Soil Pollut., 51(1-2), 1-12 (1990)
A Cahouet et al.
International journal of pharmaceutics, 242(1-2), 367-371 (2002-08-15)
The aim of the present work was to study the biodistribution of a radiolabeled lipidic nanocapsule formulation after intravenous administration in rat by scintigraphy and gamma counting. This formulation is expected to be used as anticancer agent delivery devices and
Z Piskornik et al.
Plant physiology, 50(1), 176-182 (1972-07-01)
The "bound auxin" of Zea mays, first described by Berger and Avery (Amer. J. Bot. 1944; 31: 199-203) has been purified and partially characterized. It is an indole-3-acetic acid-containing, high molecular weight, lipophilic cellulosicglucan. The indole-3-acetic acid is in ester
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实验方案

Column Packing & Separation with Sephadex® LH-20

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.

Column Packing & Separation with Sephadex® LH-20

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.

Column Packing & Separation with Sephadex® LH-20

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.

Column Packing & Separation with Sephadex® LH-20

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.

Questions

1–6 of 6 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What type of water can be used with Sephadex LH-20? Is it should be double deionized water or deionized water or distilled water?

    1 answer

    1. The manufacturer does not designate distilled or deionized water. However, a brief search of the literature reveals most publications note distilled water.

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  4. What is Sephadex LH-20, Product LH20100?

    1 answer

    1. Sephadex LH-20 is a beaded, cross-linked dextran which has been hydroxypropylated to yield a chromatographic media with both hydrophilic and lipophilic character.  Due to its dual character, LH-20 swell in water and a number of organic solvents.  The particle size and exclusion limit vary depending on the solvent used for swelling.  The swollen resin can be used for gel filtration separations of both hydrophilic and lipophilic compounds.

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  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  6. What solvents can be used with Sephadex LH-20, Product LH20100?

    1 answer

    1. Sephadex LH-20 swells in water, polar organic solvents, aqueous solvent mixtures, and mixtures of polar organic solvents.  In solvent mixtures containing both polar and non-polar organic solvents the gel takes up predominantly the polar component.   Following is a table of solvents and the approximate swelling volume.  Solvents giving a bed volume of less than 2.5 ml/g dry gel are generally not used.Solvent                           Approx. bed volume (ml/g dry gel)Dimethyl sulfoxide           4.4 - 4.6Pyridine                          4.2 - 4.4Water                             4.0 - 4.4Dimethyl formamide        4.0 - 4.4Methanol                        3.9 - 4.3Chloroform                     3.8 - 4.1Ethanol                           3.6 - 3.9Methylene dichloride       3.6 - 3.9Tetrahydrofuran              3.3 - 3.6Acetone                          2.4 - 2.6Acetonitrile                     2.2 - 2.4Benzene                         1.6 - 2.0Ethyl acetate                   1.6 - 1.8Toluene                          1.5 - 1.6

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