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Merck
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Key Documents

L0636

Sigma-Aldrich

来自 普通小麦 的凝集素（小麦）

suitable for cell culture, BioReagent, lyophilized powder

别名:

WGA, Wheat germ agglutinin

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.32

無菌

aseptically processed

品質等級

200

產品線

BioReagent

形狀

lyophilized powder

技術

cell culture | mammalian: suitable

雜質

salt, essentially free

儲存溫度

2-8°C

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相关类别

一般說明

来自 小麦 的凝集素是36kDa的二聚体糖蛋白。它属于几丁质结合蛋白家族。凝集素的糖基化前体在内质网中合成。

應用

小麦来源凝集素已用于：
  • 与N-羟基琥珀酰亚胺（NHS）染料偶联
  • 抑制核输入
  • 抑制人类宫颈癌HeLa和SiHa细胞的核孔

生化／生理作用

具有四个结合位点的小麦凝集素对N-乙酰基-d-葡糖胺和N-乙酰基-d-神经氨酸（唾液酸）残基具有特异性。凝集素也起杀真菌剂的作用。它们还具有抗营养作用。
WGA 不具有血型特异性，但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。

其他說明

高度纯化

準備報告

由亲和纯化凝集素制备偶联物。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Effects of wheat germ agglutinin on human gastrointestinal epithelium: insights from an experimental model of immune/epithelial cell interaction
Dalla P, et al.
Toxicology and Applied Pharmacology, 237, 146-153 (2009)
Super-multiplex vibrational imaging
Wei L, et al.
Nature, 544, 465-465 (2017)
Pengfei Zhang et al.
Chemical science, 13(43), 12760-12768 (2022-12-16)
Exosome analysis is a promising tool for clinical and biological research applications. However, detection and biomarker quantification of exosomes is technically challenging because they are small and highly heterogeneous. Here, we report an optical approach for imaging exosomes and quantifying
Plant lectins: occurrence, biochemistry, functions and applications
Rudiger H and Gabius HJ
Glycoconjugate Journal, 18, 589-613 (2001)
Jonathan Welch et al.
Regulatory toxicology and pharmacology : RTP, 125, 105022-105022 (2021-08-02)
The aim of the study was to use multiple in vitro assays to assess the effects of a model irritant, sodium dodecyl sulphate (SDS) (≤10 mM (0.29 %, w/v)), on an in vitro model of the airway, MucilAir™. The use of MucilAir™
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