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I3909

Sigma-Aldrich

Ionomycin calcium salt 钙盐

Ionomycin calcium salt
1 of 1 reviewers received a sample product or took part in a promotion

Ready Made Solution, from Streptomyces conglobatus, 1 mM in DMSO

别名:

钙离子载体离子霉素

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About This Item

经验公式（希尔记法）:
C41H70CaO9
CAS号:
56092-82-1
分子量:
747.07
EC號碼:
200-664-3
MDL號碼:
MFCD00083634
分類程式碼代碼:
51102829
PubChem物質ID:
329815372
NACRES:
NA.85
价格与库存信息目前不能提供

生物源

Streptomyces conglobatus

品質等級

300

化驗

≥98% (HPLC)

儲存期限

2 yr

儲存條件

(Tightly closed. )

濃度

1 mM in DMSO

顏色

colorless

mp

18.4 °C

溶解度

DMSO: 1 mM

抗生素活性譜

Gram-positive bacteria

作用方式

cell membrane | interferes

運輸包裝

wet ice

儲存溫度

−20°C

SMILES 字串

C[C@@H]1CCC(=O)O[Ca]OC(=CC(=O)[C@@H](C)C[C@@H](C)C1)[C@H](C)C[C@H](C)C\C=C\[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]2CC[C@](C)(O2)[C@H]3CC[C@](C)(O3)[C@@H](C)O

InChI

1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1

InChI 密鑰

WKRWUYKLUMMAKG-WYGBAUISSA-L

相关类别

一般說明

离子霉素(Ionomycin)是一种由密团链霉菌(Streptomyces conglobatus)产生的钙离子载体，可穿过细胞膜运输钙离子。常作为一种高效工具用以研究钙离子介导信号转导及钙离子在各种生物过程中的作用，包括细胞激活、分泌、增殖和凋亡。

离子霉素在细胞生物学研究中常用于快速可控地提高胞内钙离子浓度。进而可研究钙离子信号转导通路的下游效应，例如酶激活、激素释放和基因表达变化。离子霉素也用于研究钙离子在某些细胞过程中的作用，包括细胞死亡、免疫细胞激活和肌肉收缩。具体上，比如离子霉素可用于诱导癌细胞凋亡、研究T细胞激活、探索肌肉收缩中的钙作用。

應用

离子霉素用途：
  • 研究钙离子介导信号转导及钙离子在各种生物过程中的作用
  • 研究细胞内钙浓度、钙依赖性酶和钙内流引起的细胞反应
  • 在细胞生物学研究中快速可控地提高胞内钙离子浓度
  • 联合荧光钙离子指示剂或蛋白激酶激活剂等物质研究某些细胞机制和信号通路
离子霉素已用于：
  • 诱发中枢性脱髓鞘[1]
  • 抑制牛肾上腺TREK-1通道[2]
  • 调节人成熟B细胞的细胞分裂[3]

生化／生理作用

比A23187更有效地作为Ca2+的移动离子载体的Ca2+ 离子载体
离子霉素是一种离子渗透型抗生素，可与Pb2+和Ca2+结合，用作两种阳离子的有效移动载体。

作用机制：促进钙离子(Ca2+)跨生物膜运输

抗菌谱：对革兰氏阳性菌有抗菌活性。
离子霉素是一种离子渗透型抗生素，可与Pb2+和Ca2+结合，可用作两种阳离子的有效动态载体。离子霉素作为C2+的动态载体比A23187更有效。它是非荧光的。它用于研究跨生物膜的Ca2+转运，并诱导胚胎皮质神经元的凋亡性变性。

特點和優勢

  • 成品溶液，无需制备
  • 钙离子信号转导及钙在各种生物过程中作用的高效研究工具
  • 快速可控地调节胞内钙浓度
  • 功能多样，广泛用于细胞生物学研究

其他說明

如需了解生化试剂系列的更多信息，请填写此表。

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

188.6 °F - closed cup

閃點（°C）

87 °C - closed cup

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000206912
0000213730
0000369268
0000325568
0000206912

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K M Kim et al.
Journal of immunology (Baltimore, Md. : 1950), 148(6), 1797-1803 (1992-03-15)
The growth of a human B lymphoma cell line B104, an experimental model for mature B cells, was inhibited by ionomycin but not 12-O-tetradecanoylphorbol-13-acetate (TPA). Ionomycin inhibited B104 cells from entering into the M phase of the cell cycle without
K J Smith et al.
Brain : a journal of neurology, 117 ( Pt 6), 1351-1356 (1994-12-01)
The effects of injecting the calcium-selective ionophore, ionomycin, into myelinated tracts in the dorsal columns of adult rat spinal cords were examined electron microscopically. In vivo, ionomycin induced a primary vesicular demyelination, together with a variable degree of axonal degeneration
Youhui Si et al.
Science advances, 6(32), eaba0995-eaba0995 (2020-08-22)
The current paradigm that subunit vaccines require adjuvants to optimally activate innate immunity implies that increased vaccine reactogenicity will invariably be linked to improved immunogenicity. Countering this paradigm, nanoparticulate vaccines have been reported to act as delivery systems for vaccine
Shakti Singh et al.
PLoS pathogens, 15(8), e1007460-e1007460 (2019-08-06)
Candida auris is an emerging, multi-drug resistant, health care-associated fungal pathogen. Its predominant prevalence in hospitals and nursing homes indicates its ability to adhere to and colonize the skin, or persist in an environment outside the host-a trait unique from
Fabio Arturo Iannotti et al.
British journal of pharmacology, 176(10), 1568-1584 (2018-08-04)
Duchenne muscular dystrophy (DMD), caused by dystrophin deficiency, results in chronic inflammation and irreversible skeletal muscle degeneration. Moreover, the associated impairment of autophagy greatly contributes to the aggravation of muscle damage. We explored the possibility of using non-euphoric compounds present
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Questions

1–7 of 7 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Has Ionomycin calcium salt solution, Product No. I3909, been tested for use in Cell Culture applications?

    1 answer

    1. Sigma Aldrich has not tested this material for use in cell culture.  However, several references for use of ionomycin in cell culture applications can be found in the product information sheet (under Documents, above).

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  4. What is the concentration of the Ionomycin calcium salt solution, Product No. I3909?

    1 answer

    1. It is a 1mM solution or ~ 0.75mg/mL in DMSO.

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  5. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  7. Is Product No. I3909 (Ionomycin calcium salt solution) hygroscopic?

    1 answer

    1. Yes, this product is hygroscopic.  It is packaged under an inert gas.  It is recommended to store I3909 under an inert gas and at -20°C protected from light.

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1 of 1 reviewers received a sample product or took part in a promotion

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  1. Boston, MA
    • Reviews 2
    • Votes 2
    5 out of 5 stars.

    works

    We use it for cell culture and it works as intended.

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