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Merck
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文件

I1643

Sigma-Aldrich

焦磷酸酶，无机 来源于面包酵母（酿酒酵母）

powder, ≥500 units/mg protein (E1%/280)

别名:

无机焦磷酸酶, 焦磷酸盐磷酸水解酶

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About This Item

CAS号:
9024-82-2
EC 号:
3.6.1.1 (BRENDA, IUBMB)
EC號碼:
232-784-7
MDL號碼:
MFCD00131379
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

powder

品質等級

300

比活性

≥500 units/mg protein (E1%/280)

分子量

71 kDa

儲存溫度

−20°C

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一般說明

分子量：71kDa（由分子量为 32-35kDa 的两个相同亚基组成的同型二聚体）
吸光系数：E1% = 14.5 (280nm)
pI：4.75
这种普遍存在的酶可驱动生成焦磷酸盐的代谢反应，因为这些反应的平衡常数通常接近整数。催化的机理在文献中有所记述。无机焦磷酸酶是一种金属蛋白酶，达到最大活性需要 Mg2+ 的参与。虽然无机焦磷酸盐的水解反应在 Mg2+ 存在下具有特异性，但是如果存在锌 ADP 和 ATP 均会被水解。

應用

无机焦磷酸酶 (PPase) 是一种普遍存在的酶，可催化 PPi + H2O → 2Pi。
它在蛋白质、RNA 和 DNA 合成中发挥着重要作用。
焦磷酸酶已被用于：
  • 作为反应缓冲液的组分用于氨酰化的测定，以对掺入tRNA的[3H]L-甲硫氨酸进行定量。
  • 作为PCR混合物的组分。
焦磷酸酶已被用于：
作为反应缓冲液的组分用于氨酰化的测定，以对掺入tRNA的[3H]L-甲硫氨酸进行定量。
作为PCR混合物的组分

單位定義

在pH 7.2，25 °C条件下，一个单位可在每分钟释放1.0μmole无机正磷酸盐。

外觀

冻干粉末，含80-90%缓冲盐

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Virginia P Ronchi et al.
The Journal of biological chemistry, 292(44), 18006-18023 (2017-09-20)
To understand the mechanism for assembly of Lys
Laura Pedró-Rosa et al.
Journal of biomolecular screening, 20(1), 122-130 (2014-08-29)
Improved therapies for the treatment of Trypanosoma brucei, the etiological agent of the neglected tropical disease human African trypanosomiasis, are urgently needed. We targeted T. brucei methionyl-tRNA synthetase (MetRS), an aminoacyl-tRNA synthase (aaRS), which is considered an important drug target
Enzymatic chemiluminescence assay for inorganic pyrophosphate.
Vuokko Jansson et al.
Analytical biochemistry, 304(1), 135-137 (2002-04-24)
Jin He et al.
Annals of neurology, 93(2), 244-256 (2022-09-12)
Despite the increasing number of genes associated with Charcot-Marie-Tooth (CMT) disease, many patients currently still lack appropriate genetic diagnosis for this disease. Autosomal dominant mutations in aminoacyl-tRNA synthetases (ARSs) have been implicated in CMT. Here, we describe causal missense mutations
Musleh M Muthana et al.
Chemical communications (Cambridge, England), 48(21), 2728-2730 (2012-02-07)
A promiscuous UDP-sugar pyrophosphorylase (BLUSP) was cloned from Bifidobacterium longum strain ATCC55813 and used efficiently with a Pasteurella multocida inorganic pyrophosphatase (PmPpA) with or without a monosaccharide 1-kinase for one-pot multienzyme synthesis of UDP-galactose, UDP-glucose, UDP-mannose, and their derivatives. Further
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