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H8889

Sigma-Aldrich

Histopaque®-1077 Hybri-Max™

liquid, sterile-filtered, BioReagent, suitable for hybridoma

别名:

Histopaque 1077

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About This Item

UNSPSC代码:
12352201
NACRES:
NA.75

无菌性

sterile-filtered

产品线

BioReagent

表单

liquid

技术

cell culture | hybridoma: suitable
single cell analysis: suitable

杂质

endotoxin, tested

密度

1.077 at 25 °C (relative density)(lit.)

储存温度

2-8°C

相关类别

一般描述

Histopaque®-1077是包含多蔗糖和泛影酸钠的溶液，密度调整到1.077 +/- 0.001 g/cm3。这一培养基帮助来自小体积全血的活的淋巴细胞快速恢复。这一技术适合于调节淋巴溶解和人类淋巴细胞抗原（HLA）2分型的细胞。它还可用作T-、B-和“空”淋巴细胞计数前的起始分离步骤。用来建立密度培养基，用于淋巴细胞和其他单核细胞的纯化。

应用

Histopaque®-1077 Hybri-Max™已用于分离：
  • 外周血单个核细胞（PBMC）与肝素化血，梯度离心法
  • 来自外周血的免疫细胞（淋巴细胞）
  • PBMC与血液

法律信息

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H317,H334

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBM0089
RNBL7498
RNBL3437
RNBL2041
RNBK6706

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Lymphoblastoid cell lines (LCLs) are generated by transforming primary B cells with Epstein-Barr virus (EBV) and are used extensively as model systems in viral oncology, immunology, and human genetics research. In this study, we characterized single-cell transcriptomic profiles of five
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Radiotherapy (RT) traditionally has been used for local tumor control in the treatment of cancer. The recent discovery that radiotherapy can have anticancer effects on the immune system has led to recognition of its ability to sensitize the tumor microenvironment
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Deciphering the molecular pathogenesis of virally induced cancers is challenging due, in part, to the heterogeneity of both viral gene expression and host gene expression. Epstein-Barr virus (EBV) is a ubiquitous herpesvirus prevalent in B-cell lymphomas of immune-suppressed individuals. EBV
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实验方案

Histopaque® Density Gradient Media Troubleshooting Guide

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

Histopaque® 密度梯度介质故障排除指南

本疑难排查指南针对使用 Histopaque 时观察到的最常见错误来源提出解决方案，但这并不代表本指南是全面无遗的。

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