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一般說明
Percoll®是一种用于细胞、病毒和亚细胞颗粒密度梯度离心的经典培养基。它由直径为15-30 nm（在水中为23％ w/w）并涂覆有聚乙烯吡咯烷酮（PVP）的胶体二氧化硅颗粒组成。PVP涂层使产品完全无毒，非常适合与生物材料一起使用。由于其颗粒大小的不均匀性，沉积以不同的速率发生，自发地在1.0-1.3 g/ml范围内产生非常光滑的等距梯度。
應用
Percoll®已用于以下材料的离心分离：
- 新生大鼠心室心肌细胞
- 血液样本中的白细胞
- 小管周围肌样（PM）细胞
Percoll由粒径为15-30nm(在水中为23% w/w)的硅胶颗粒组成，这些颗粒被聚乙烯吡咯烷酮(PVP)包覆。 它被用来建立无毒低粘度，低渗透压密度梯度，可用于分离细胞，细胞器和病毒。它已被用来分离 大肠杆菌的内外膜泡。 Percoll在整个梯度过程中都是等渗的，无毒，并且很容易从纯化的材料中去除。
特點和優勢
- 低渗透压允许对生理条件进行精确调整，而不会受到介质的明显干扰。
- 与活细胞和病毒兼容，并允许分离和恢复完整的，完全活跃的系统。
- 对生物膜不渗透，在离心过程中不改变颗粒的浮力密度。
- 在离心过程中自发形成的梯度允许在离心管中混合大量样品。
- 低粘度导致快速形成梯度和颗粒分离。
- 大多数沉降系数大于60S的生物颗粒可以在Percoll梯度中成功分离。
法律資訊
Percoll is a registered trademark of Cytiva
相關產品
产品编号
说明
价格
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
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实验方案
为了配置Percoll梯度液，必须先用生理盐水或细胞培养基将Percoll调至生理性渗透压。
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
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