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表单
liquid
包装
pkg of 1 kit
制造商/商品名称
Cytiva RPN3682
运输
wet ice
储存温度
2-8°C
一般描述
AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star detection reagent allows for chemiluminescent detection of Alkaline Phosphatase labelled nucleic acids.
Sufficient reagent for 2500cm² of membrane. Can be used in conjunction with AlkPhos Direct Labeling Module (RPN3680), Amersham AlkPhos Direct Hybridization Buffer (RPN3688) and Hybond/ Nytran membranes.
Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500 cm2 or 5000 cm2 membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star or ECF™ detection reagents.
Sufficient reagent for 2500cm² of membrane. Can be used in conjunction with AlkPhos Direct Labeling Module (RPN3680), Amersham AlkPhos Direct Hybridization Buffer (RPN3688) and Hybond/ Nytran membranes.
Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500 cm2 or 5000 cm2 membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star or ECF™ detection reagents.
应用
CDP-Star® has been used to label and quantify genomic DNA by Southern blotting.
特点和优势
- Probes are labelled during a 30 minute reaction, resulting in probes that can be used without purification.
- Hybridization stringency can be controlled by temperature as well as salt concentration. Detection is rapid, taking only 30 minutes from completion of hybridization to application of detection reagent.
- Detection is rapid, taking only 30 minutes from completion of hybridization to application of detection reagent.
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
CDP-Star is a registered trademark of Tropix, Inc.
ECF is a trademark of Cytiva
警示用语：
Danger
危险声明
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
其他客户在看
Daniel de Melo Pereira et al.
Frontiers in bioengineering and biotechnology, 8, 554565-554565 (2020-11-17)
Biomineralized collagen with intrafibrillar calcium phosphate mineral provides an excellent mimic of the composition and structure of the extracellular matrix of bone, from nano- to micro-scale. Scaffolds prepared from this material have the potential to become the next-generation of synthetic
商品
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
描述被分子生物学家用于检测转印膜上蛋白质和核酸的方法的背景和实验方案。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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