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11585614910

Roche

DIG-High Prime DNA标记及检测启动装II

greener alternative

sufficient for 12 labeling reactions (10 ng to 3 μg per assay), sufficient for 24 blots (blots of 100 cm2)

别名:

DIG系统, DNA标记

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About This Item

UNSPSC代码:
41105500

用途

sufficient for 12 labeling reactions (10 ng to 3 μg per assay)
 sufficient for 24 blots (blots of 100 cm2)

质量水平

100

制造商/商品名称

Roche

环保替代产品特性

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

环保替代产品分类

Aligned,

储存温度

−20°C

相关类别

一般描述

DIG-High Prime DNA标记和检测起始试剂盒II是一种便捷试剂盒，用于通过地高辛(DIG)-11-脱氧尿苷三磷酸(dUTP)随机引物标记DNA模板，对DIG标记杂交进行碱不稳定性和化学发光检测。该试剂盒的组装就是以便捷性为宗旨，提供即用型的CSPD并带有可用于简易应用的滴液装置、成品的封闭溶液以及DIG Easy Hyb颗粒。DIG-High Prime混合物包括稳定的Klenow酶、核苷酸、引物和反应缓冲液——所有成分均溶于一种方便的试剂中。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品为一种安全的化学品。  DIG系统是灵敏且具有成本效益的方案，可替代核酸放射性标记和检测。有许多已发表论文证明了DIG系统的通用性，因此，使用放射性标记不再是标记用于杂交的DNA的唯一选择。

应用

DIG-High Prime DNA标记和检测起始试剂盒II已用于多种杂交技术：
  • Southern印迹
  • Nrthern印迹
  • 斑点印迹
  • 菌落和噬菌斑杂交
  • 所有类型的过滤杂交
  • 总基因组DNA的单拷贝基因检测，甚至可检测高度复杂的生物体样品，如人类、大麦和小麦

包装

1个试剂盒包含7种组分。

原理

DIG-High Prime DNA标记及检测启动装II使用地高辛（DIG），一种类固醇半抗原，对DNA探针进行标记用于杂交以及后续通过酶免疫检测的化学发光检测。“随机结合的”DNA标记方法首先由Feinberg和Vogelstein开发，基于将所有可能序列的寡核苷酸杂交至待标记的变性DNA。起始加入的DNA仅作为标记DNA的合成模板并在反应过程中不会降解，使得利用该方法能够对最低量的DNA（10 ng）进行标记。互补的DNA链通过Klenow聚合酶利用随机的寡核苷酸的3′-OH端作为引物进行合成。存在与反应中标记了地高辛的修饰后脱氧核糖核苷三磷酸被插入至新合成的互补DNA链中。

其他说明

仅用于生命科学研究。不可用于诊断。

仅试剂盒组分

产品编号
说明
  • DIG-High Prime 5x concentrated
  • DIG-labeled Control DNA, pBR328 (linearized with Bam HI) 5 μg/ml
  • DNA Dilution Buffer
  • Anti-Digoxigenin-AP Conjugate antibody
  • CSPD ready-to-use
  • Blocking Solution 10x concentrated
  • DIG Easy Hyb Granules

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H315,H319

危险分类

Eye Irrit. 2 - Skin Irrit. 2

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

does not flash

闪点(°C)

does not flash

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
59457520
56477220
35941221
35941220
27394020

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International journal of genomics, 2014, 921950-921950 (2014-09-10)
The Delta-12 oleate desaturase gene (FAD2-1), which converts oleic acid into linoleic acid, is the key enzyme determining the fatty acid composition of seed oil. In this study, we inhibited the expression of endogenous Delta-12 oleate desaturase GmFad2-1b gene by
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The potent antimalarial drug artemisinin has a high cost, since its only viable source to date is Artemisia annua (0.01-0.8% DW). There is therefore an urgent need to design new strategies to increase its production or to find alternative sources.
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Malaria is causing more than half of a million deaths and 214 million clinical cases annually. Despite tremendous efforts for the control of malaria, the global morbidity and mortality have not been significantly changed in the last 50 years. Artemisinin, extracted
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Untargeted and uncontrolled gene delivery is a major cause of gene therapy failure. This study aimed to define efficient and safe tissue-selective targeted gene therapy approaches for delivering genes into keratocytes of the cornea in vivo using a normal or
Yu Du et al.
Tetrahedron, 67(35), 6568-6575 (2011-08-27)
The apoptolidins are 20/21-membered macrolides produced by Nocardiopsis sp. FU40. Several members of this family are potent and remarkably selective inducers of apoptosis in cancer cell lines, likely via a distinct mitochondria associated target. To investigate the biosynthesis of this
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