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GE17-5279-02

Glutathione Seph High Performance

Cytiva 17-5279-02, pack of 100 mL

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About This Item

UNSPSC代码:
23151817
NACRES:
NA.56

ligand

glutathione

包装

pack of 100 mL

制造商/商品名称

Cytiva 17-5279-02

基质

highly cross-linked 6% agarose

平均零件尺寸

34 μm

cleaning

3-12

工作范围

3-12

容量

>7 mg/mL binding capacity(medium)

相关类别

一般描述

Glutathione Sepharose™ High Performance is an affinity medium for high resolution purification of Glutathione S transferase (GST) tagged proteins.

应用

Glutathione Sepharose™ High Performance is used for the purification of GST-tagged Proteins from bacterial lysates and other S-transferases or glutathione-dependent Proteins. GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function.

特点和优势

  • 34 μm bead size for improved efficiency and resolution
  • Fast, one-step purification of GST-tagged Proteins and other glutathione S-trasferase or glutathione-dependent Proteins
  • Mild conditions during the purification process preserves Protein antigenicity and function

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

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实验方案

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

GSTrap™ Columns for Purification

GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.

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