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GE17-0756-01

Glutathione Sepharose™ 4B

Cytiva 17-0756-01, pack of 10 mL

别名:

affinity chromatography resin, GST-tagged proteins purification,

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

ligand

glutathione

包裝

pack of 10 mL

製造商／商標名

Cytiva 17-0756-01

基質

4% agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning in place

4-13

工作範圍

4-13

容量

>25 mg binding capacity (horse liver GST/ml medium)

相关类别

一般說明

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

特點和優勢

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

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Xin Zhou et al.
Nature communications, 11(1), 2674-2674 (2020-05-31)
Increasing evidence indicates that guidance molecules used during development for cellular and axonal navigation also play roles in synapse maturation and homeostasis. In C. elegans the netrin receptor UNC-40/DCC controls the growth of dendritic-like muscle cell extensions towards motoneurons and
Amandine Cartier-Michaud et al.
PLoS genetics, 13(6), e1006803-e1006803 (2017-06-16)
Spermatogenesis is a dynamic process that is regulated by adhesive interactions between germ and Sertoli cells. Germ cells express the Junctional Adhesion Molecule-C (JAM-C, encoded by Jam3), which localizes to germ/Sertoli cell contacts. JAM-C is involved in germ cell polarity
Ye Guan et al.
eLife, 7 (2018-04-06)
Single-cell analysis is pivotal to deciphering complex phenomena like heterogeneity, bistability, and asynchronous oscillations, where a population ensemble cannot represent individual behaviors. Bulk cell-free systems, despite having unique advantages of manipulation and characterization of biochemical networks, lack the essential single-cell
Gerard H Pieper et al.
Developmental cell, 53(1), 27-41 (2020-02-29)
Eukaryotic genomes are organized within the nucleus through interactions with inner nuclear membrane (INM) proteins. How chromatin tethering to the INM is controlled in interphase and how this process contributes to subsequent mitotic nuclear envelope (NE) remodeling remains unclear. We have
Amandine Draime et al.
Biochimica et biophysica acta, 1861(5), 534-542 (2018-02-23)
Understanding how the activity of transcription factors like HOX proteins is regulated remains a widely open question. In a recent screen for proteins interacting with HOXA1, we identified a PRDM protein family member, PRDM14, which is known to be transiently
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商品

Troubleshooting of Problems Associated with the Expression and Growth of GST-tagged Proteins

This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.

Purification using GST SpinTrap™

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

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实验方案

GST Detection Module with CDNB Enzymatic Assay

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

GST Detection Module with CDNB Enzymatic Assay

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

GST Detection Module with CDNB Enzymatic Assay

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

GST Detection Module with CDNB Enzymatic Assay

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

查看所有结果

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