推荐产品
ligand
glutathione and 10-carbon linker arm
包裝
pack of 100 mL
製造商/商標名
Cytiva 17-5132-02
基質
highly cross-linked 4% agarose
平均直徑
90 μm
cleaning
3-12
工作範圍
3-12
容量
>10 mg/mL binding capacity
一般說明
Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.
特點和優勢
- Fast, one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- For scale-up due to high flow properties
- Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
其他客户在看
商品
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
实验方案
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.
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