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Merck
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文件

G4510

Millipore

谷胱甘肽−琼脂糖

lyophilized powder

别名:

谷胱甘肽-琼脂糖树脂, GSH-琼脂糖, S-连接谷胱甘肽琼脂糖

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About This Item

MDL號碼:
MFCD00131200
分類程式碼代碼:
41106500
NACRES:
NA.56

形狀

lyophilized powder

分析物化學類別

proteins (GST)

標籤範圍

≥10 μmol per mL gel

技術

immunoprecipitation (IP): suitable
protein purification: suitable

基質

4% cross-linked beaded agarose

基質活化

epoxy

基質結合

sulfur

基質墊片

12 atoms (10 carbon)

儲存溫度

−20°C

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一般說明

谷胱甘肽硫转移酶(GST)是由天然真核细胞生产的26 KDa分子量蛋白。它属于II相解毒酶家族的一员。谷胱甘肽-琼脂糖树脂由谷胱甘肽通过硫与环氧活化的4%交联琼脂糖珠相连形成12个原子（10碳）的间隔臂。本品为冻干粉末，添加了乳糖稳定剂。

應用

谷胱甘肽琼脂糖树脂用于：
  • 纯化谷胱甘肽硫转移酶(GST)融合蛋白，生成多克隆抗体M4P
  • 通过孵育上清液组分，纯化全长p53蛋白(aa 1-393)及其DNA结合域(aa 94-288)
  • 融合蛋白的亲和纯化

利用谷胱甘肽-琼脂糖亲和层析技术，可以快速、温和、非变性和高度选择性地纯化含有谷胱甘肽结合序列的蛋白质，如谷胱甘肽S-转移酶(GST)、谷胱甘肽过氧化物酶和乙二醛酶I。

生化／生理作用

谷胱甘肽硫转移酶(GST)属于多功能解毒同工酶超家族成员，在细胞信号传导中起重要作用。由于其特定同工酶在多种肿瘤中过表达，并且可能参与神经退行性疾病、多发性硬化症和哮喘等其他疾病的发生发展，GST成为前景可期的治疗靶标。

特點和優勢

  • 提供快速、温和、非变性、高度选择性的谷胱甘肽结合酶纯化。
  • 可在-20 °C恰当干燥保存2年。
  • 载量：每mL树脂可结合5-10 mg谷胱甘肽S-转移酶

數量

1g粉末可溶胀至 ≥10 ml凝胶

外觀

用乳糖稳定化的冻干粉

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Wenrong Tan et al.
PLoS genetics, 14(4), e1007336-e1007336 (2018-04-17)
Drought is a major threat to plant growth and crop productivity. The phytohormone abscisic acid (ABA) plays a critical role in plant response to drought stress. Although ABA signaling-mediated drought tolerance has been widely investigated in Arabidopsis thaliana, the feedback
Magdalena M Grabowska et al.
Molecular endocrinology (Baltimore, Md.), 28(6), 949-964 (2014-05-08)
Androgen receptor (AR) action throughout prostate development and in maintenance of the prostatic epithelium is partly controlled by interactions between AR and forkhead box (FOX) transcription factors, particularly FOXA1. We sought to identity additional FOXA1 binding partners that may mediate
P G Board et al.
The Journal of biological chemistry, 275(32), 24798-24806 (2000-04-28)
A new class of glutathione transferases has been discovered by analysis of the expressed sequence tag data base and sequence alignment. Glutathione S-transferases (GSTs) of the new class, named Omega, exist in several mammalian species and Caenorhabditis elegans. In humans
Dustin L Johnson et al.
Journal of bacteriology, 190(8), 2972-2980 (2008-02-19)
Chlamydophila pneumoniae is a gram-negative obligate intracellular bacterial pathogen that causes pneumonia and bronchitis and may contribute to atherosclerosis. The developmental cycle of C. pneumoniae includes a morphological transition from an infectious extracellular elementary body (EB) to a noninfectious intracellular
Samie R Jaffrey et al.
Proceedings of the National Academy of Sciences of the United States of America, 99(5), 3199-3204 (2002-02-28)
The specificity of the reactions of nitric oxide (NO) with its neuronal targets is determined in part by the precise localizations of neuronal NO synthase (nNOS) within the cell. The targeting of nNOS is mediated by adapter proteins that interact
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蛋白质Pull-Down

利用亲和力或GST沉降、串联亲和纯化（TAP）和免疫共沉淀法研究体外蛋白-蛋白相互作用所需的pull-down检测方法、试剂和实验方案。

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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