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一般說明
Ficin from fig tree latex exists in multiple isoforms. The isoforms are prone to autolysis at varying temperature and tend to degrade during long term storage. It is a sulfhydryl protease with eight cysteine residues and is stabilized by three disulfide bridges.
消光系数： E1% = 21.0 (280 nm)
pI: 9.0
pI: 9.0
應用
Ficin from fig tree latex has been used:
- in the electron paramagnetic resonance (EPR) and steady-kinetic measurements for assessing its peroxidase functionality
- for the digestion of eye tissue sections prior to immunostaining and immunofluorescence
生化／生理作用
无花果蛋白酶（Ficin）被归类为硫醇蛋白酶。它的活性位点上含有单个反应性半胱氨酸。其活性位点的氨基酸同源性与木瓜蛋白酶相似。无花果蛋白酶将切割Gly、Ser、Thr、Met、Lys、Arg、Tyr、Ala、Asn和Val羧基侧的蛋白质。据报道，显色底物pGlu-Phe-Leu-p-硝基苯胺的 Km 为0.43 mM。无花果蛋白酶可被碘乙酰胺、碘乙酸、N-乙基马来酰亚胺、氯化汞、DFP（二异丙基氟磷酸酯）、TLCK（Na-对甲苯磺酰基-赖氨酸氯甲基酮）和TPCK（N-甲苯磺酰基-L-苯丙氨酸氯甲基酮）抑制。无花果蛋白酶可用于以高得率产生小鼠IgG1的F (ab′)2 片段。
單位定義
在 pH 7.0 和 37°C 下，当在最终体积为 10mL（1cm 光程）的溶液中测定酪蛋白的 TCA 可溶性产物时，一单位每分钟产生的 ΔA280 为 1.0。
外觀
2.0M NaCl 和 0.03M 半胱氨酸中的悬浮液 (pH 5.0)
準備報告
2 次结晶
抑制劑
产品编号
说明
价格
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
其他客户在看
Transgenic expression of leukemia inhibitory factor (LIF) blocks normal vascular development but not pathological neovascularization in the eye
Ash J, et al.
Molecular Vision, 11, 298-308 (2005)
Lens-specific VEGF-A expression induces angioblast migration and proliferation and stimulates angiogenic remodeling
Ash JD and Overbeek PA
Developmental Biology, 223(2), 383-398 (2000)
Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex
Zare H, et al.
Phytochemistry, 87, 16-22 (2013)
Yufang Yang et al.
Scientific reports, 7, 43141-43141 (2017-02-23)
Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we found sufficient proofs for
Kamsagara Basavarajappa Devaraj et al.
Journal of agricultural and food chemistry, 56(23), 11417-11423 (2008-11-11)
Ficin (EC 3.4.22.3), a cysteine proteinase isolated from the latex of a Ficus tree, is known to occur in multiple forms. Although crude ficin is of considerable commercial importance, ficin as such has not been fully characterized. A major ficin
实验方案
This procedure may be used for all Ficin products.
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