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Merck
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F4165

Sigma-Aldrich

无花果蛋白酶 来源于无花果树乳胶

powder, ≥0.1 unit/mg solid

别名:

Debricin, Ficain, higueroxyl delabarre

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About This Item

CAS号:
9001-33-6
EC 号:
3.4.22.3 (BRENDA, IUBMB)
EC號碼:
232-599-1
MDL號碼:
MFCD00081597
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

fig tree (latex)

形狀

powder

比活性

≥0.1 unit/mg solid

分子量

23.8 kDa

儲存溫度

−20°C

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相关类别

一般說明

消光系数： E1% = 21.0 (280 nm)
pI: 9.0

生化／生理作用

无花果蛋白酶（Ficin）被归类为硫醇蛋白酶。它的活性位点上含有单个反应性半胱氨酸。其活性位点的氨基酸同源性与木瓜蛋白酶相似。无花果蛋白酶将切割Gly、Ser、Thr、Met、Lys、Arg、Tyr、Ala、Asn和Val羧基侧的蛋白质。据报道，显色底物pGlu-Phe-Leu-p-硝基苯胺的 Km 为0.43 mM。无花果蛋白酶可被碘乙酰胺、碘乙酸、N-乙基马来酰亚胺、氯化汞、DFP（二异丙基氟磷酸酯）、TLCK（Na-对甲苯磺酰基-赖氨酸氯甲基酮）和TPCK（N-甲苯磺酰基-L-苯丙氨酸氯甲基酮）抑制。无花果蛋白酶可用于以高得率产生小鼠IgG1的F (ab′)2 片段。

單位定義

一个酶活性单位是指在测量酪蛋白的TCA可溶性产物时，在终体积为10ml（1cm光路）、37°C、pH 7.0条件下每分钟产生1.0的ΔA280 所需的酶量。
该酶溶于1 M磷酸钾缓冲液中，pH 7.0 (0.25 mg/ml)，得到澄清溶液。

基底

产品编号
说明
价格

抑制劑

产品编号
说明
价格

象形圖

Health hazardExclamation mark
GHS08,GHS07

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书(COA)

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胃蛋白酶 lyophilized (salt-free), ~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)

Roche

10108057001

胃蛋白酶

Yufang Yang et al.
Scientific reports, 7, 43141-43141 (2017-02-23)
Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we found sufficient proofs for
Kamsagara Basavarajappa Devaraj et al.
Journal of agricultural and food chemistry, 56(23), 11417-11423 (2008-11-11)
Ficin (EC 3.4.22.3), a cysteine proteinase isolated from the latex of a Ficus tree, is known to occur in multiple forms. Although crude ficin is of considerable commercial importance, ficin as such has not been fully characterized. A major ficin
S Fadýloğlu
Die Nahrung, 45(2), 143-146 (2001-05-31)
Fig tree latex (ficin) was immobilized on Celite by adsorption. The free and immobilized ficin were utilized in the production of teleme (a Turkish milk product). After immobilization, the optimal temperature of ficin was shifted from 60 to 80 degrees
Andrey V Sorokin et al.
Polymers, 14(11) (2022-06-11)
This work aims to synthesize graft copolymers of chitosan and N-vinylimidazole (VI) with different compositions to be used as matrices for the immobilization of cysteine proteases-bromelain, ficin, and papain. The copolymers are synthesized by free radical solution copolymerization with a
Daniel Janvier et al.
Transfusion, 53(9), 1948-1955 (2012-12-18)
Rh proteins and the Wr(b) antigen, which results from an interaction between Band 3 and glycophorin A, are the most common targets for warm immunoglobulin (Ig)G autoantibodies. Apart from autoanti-Di(b) , a scarce specificity, IgG warm autoantibodies specific for Band
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实验方案

Enzymatic Assay of Ficin (EC 3.4.22.3)

This procedure may be used for all Ficin products.

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