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生物源
Serratia marcescens
品質等級
重組細胞
expressed in E. coli
等級
ultrapure grade
化驗
≥99% (SDS-PAGE)
形狀
buffered aqueous glycerol solution
分子量
30 kDa
濃度
≥250 units/μL
應用
research use
運輸包裝
wet ice
儲存溫度
−20°C
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應用
用于去除蛋白样品中的核酸。
该酶已用于一项检测不受Benzonase核酸酶降解的Tf1 RNA量的检测中。粟酒裂殖酵母的Tf1元件是含有长末端重复序列的反转录转座子，可编码功能性蛋白酶、逆转录酶和整合酶蛋白。它还用于从细胞裂解物中获得的核颗粒和核提取物的分馏。
超纯的Benzonase核酸酶，或来自粘质沙雷氏菌的内切核酸酶，已用于一项评估十二烷基苯磺酸钠和消泡剂对粘质沙雷氏菌内切核酸酶活性影响的研究中。来自粘质沙雷氏菌的内切核酸酶已用于一项探究染色质亚基结构的研究中。
生化／生理作用
Benzonase®是一种来自粘质沙雷氏菌的基因工程改造内切核酸酶。该蛋白质是30kDa亚基的二聚体，带有两个必需的二硫键。该内切核酸酶可攻击并降解所有形式的DNA和RNA（单链的、双链的、线性的和环化的），并可在一系列宽泛的操作条件下具有高效性。它可将核酸完全消化为长度为3至5个碱基的5′-单磷酸末端寡核苷酸。这是从重组蛋白中去除核酸的理想选择。它还可用于需要完全消化核酸的应用。它还可降低蛋白提取物的粘度并防止细胞成团。用这种酶对蛋白样品进行预处理可通过去除任何结合的核酸而提高它在2D凝胶电泳中的分辨率。最适的酶活pH为8.0-9.2。
消化天然或热变性的DNA和RNA。
單位定義
在pH 8.0，37 °C条件下，一个单位的酶在30分钟内可将超声的鲑鱼精子DNA消化成等同于1.0 ΔA260的酸可溶寡核苷酸（2.625 ml反应体积）。
外觀
在含有20 mM Tris HCl, pH 8.0, 2 mM MgCl2及20 mM NaCl的50%甘油中的溶液。
法律資訊
Benzonase®核酸酶由德国达姆施塔特默克公司和/或其附属公司提供。
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
其他客户在看
Laure Teysset et al.
Journal of virology, 77(9), 5451-5463 (2003-04-15)
The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. Although these proteins are known to be necessary for protein processing, reverse transcription, and integration, respectively, the function of
P Friedhoff et al.
Protein expression and purification, 5(1), 37-43 (1994-02-01)
Overproduction of the extracellular Serratia marcescens nuclease in Escherichia coli results in aggregation and sequestration of a large amount of the protein in inclusion bodies. Only a relatively small amount is secreted into the medium from which it can be
Jyoti Garg et al.
Current biology : CB, 29(14), 2371-2379 (2019-07-10)
Mediator is a large protein complex required for basal and regulated expression of most RNA polymerase II (RNAP II)-transcribed genes, in part due to its interaction with and phosphorylation of the conserved C-terminal domain (CTD) of Rpb1 [1, 2]. Mediator
Hung Ho-Xuan et al.
Nucleic acids research, 48(18), 10368-10382 (2020-09-22)
Circular RNAs (circRNAs) encompass a widespread and conserved class of RNAs, which are generated by back-splicing of downstream 5' to upstream 3' splice sites. CircRNAs are tissue-specific and have been implicated in diseases including cancer. They can function as sponges
Daria V Babushok et al.
Human mutation, 28(6), 527-539 (2007-02-20)
Long interspersed nucleotide element (LINE)-1 retrotransposon (L1) has emerged as the largest contributor to mammalian genome mass, responsible for over 35% of the human genome. Differences in the number and activity levels of L1s contribute to interindividual variation in humans
商品
This page lists nine frequently asked questions and answers about Benzonase® Nuclease.
This page lists nine frequently asked questions and answers about Benzonase® Nuclease.
本页列出了有关 Benzonase® 核酸酶的九个常见问题及其解答。
This page lists nine frequently asked questions and answers about Benzonase® Nuclease.
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