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Merck
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70664

Millipore

Benzonase® Nuclease

Purity > 99%, Effective viscosity reduction and removal of nucleic acids from protein solutions

别名:

内切核酸酶 来源于粘质沙雷氏菌

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About This Item

CAS号:
9025-65-4
EC 号:
3.1.30.2 (BRENDA, IUBMB)
MDL號碼:
MFCD00131010
分類程式碼代碼:
12352202
NACRES:
NA.54

product name

Benzonase®核酸酶，纯度>99%, Effective viscosity reduction and removal of nucleic acids from protein solutions

生物源

Serratia marcescens

品質等級

300

重組細胞

expressed in E. coli

化驗

>99% (SDS-PAGE)

形狀

buffered aqueous glycerol solution

製造商／商標名

Novagen®

儲存條件

OK to freeze

濃度

25-29 units/μL

雜質

<0.25 EU/kU Total endotoxin

應用

research use

運輸包裝

wet ice

儲存溫度

−20°C

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相关类别

一般說明

Benzonase® 核酸酶是一种用基因工程方法从粘质沙雷氏菌获得的核酸内切酶。它降解所有形式的DNA和RNA（单链、双链、线性和环状），而没有蛋白水解活性。它能够在较大范围的条件下工作，并且具有极高的比活度。该酶可将核酸完全消化为长度为2至5个碱基（低于杂交极限）的 5′-单磷酸末端寡核苷酸，特别适合于重组蛋白中核酸的去除，从而满足FDA有关核酸污染的指导原则。Benzonase可以快速水解核酸的能力使其特别适用于降低粘度，以减少加工时间，提高蛋白质产量。例如，该酶与BugBuster和PopCulture蛋白质提取试剂兼容，因此可以与这些试剂一起添加，以消除大肠杆菌提取物中的粘度并去除核酸。

该酶包括两个30kDa的亚基。它在pH值6至10，温度0-42°C 起作用，并需要1-2mM Mg2+激活。该酶在离子和非离子型洗涤剂、还原剂、PMSF（1 mM）、EDTA（1 mM）和尿素（相对活性取决于特定条件）的存在下也具有活性。>150 mM单价阳离子、>100 mM磷酸盐、>100 mM硫酸铵或>100 mM盐酸胍能抑制其活性。

可提供超纯Benzonase 核酸酶（>99%，SDS-PAGE）和纯Benzonase核酸酶（>90%），标准浓度为25-29 U/µl，高浓度（HC）为250U/lµ。两种制剂均不含可检测的蛋白酶，并且比活度为> 1 × 106U/mg蛋白。纯度> 99％ 的产品经过内毒素测试，并含有

总内毒素含量：>< 0.25 EU/1000 units. The product is supplied in 50% glycerol. Store at -20&#176;C.

纯度：< 0.25 EU/1,000 units. Purity: >99%（SDS-PAGE法）。
有效降低粘度并从蛋白质溶液中去除核酸

應用

用于去除蛋白样品中的核酸。

生化／生理作用

消化天然或热变性的DNA和RNA。

警告

毒性：标准处理（A）

單位定義

一个单位定义为在 30 分钟内导致ΔA₂₆₀为1.0的酶量，这对应于37 µg DNA的完全消化。

法律資訊

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

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Erika C Urdaneta et al.
Methods (San Diego, Calif.), 178, 72-82 (2019-10-07)
Post-transcriptional regulation of gene expression in cells is facilitated by formation of RNA-protein complexes (RNPs). While many methods to study eukaryotic (m)RNPs rely on purification of polyadenylated RNA, other important regulatory RNA classes or bacterial mRNA could not be investigated
Thach H Chu et al.
PLoS pathogens, 16(2), e1008083-e1008083 (2020-02-25)
Antibody functions such as neutralization require recognition of antigen by the Fab region, while effector functions are additionally mediated by interactions of the Fc region with soluble factors and cellular receptors. The efficacy of individual antibodies varies based on Fab
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American journal of physiology. Heart and circulatory physiology, 319(1), H109-H122 (2020-05-23)
Although cell therapy-mediated cardiac repair offers promise for treatment/management of heart failure, lack of fundamental understanding of how cell therapy works limits its translational potential. In particular, whether reparative cells from failing hearts differ from cells derived from nonfailing hearts
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The lateral habenula (LHb) is an epithalamic brain structure critical for processing and adapting to negative action outcomes. However, despite the importance of LHb to behavior and the clear anatomical and molecular diversity of LHb neurons, the neuron types of
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Viral vectors are essential tools for the study of neural circuits, with glycoprotein-deleted rabies viruses being widely used for monosynaptic retrograde tracing to map connectivity between specific cell types in the nervous system. However, the use of rabies virus is
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