The reported molecular weight is between 10-15 million Daltons before activation (nicking), primarily as double-stranded DNA. A double-stranded DNA of 1 kb in length has a molecular weight of 660 kiloDalton. A size of 15 million daltons is equivalent to 15,000 kilodaltons. When multiplied by (1kb/660 kilodaltons), it equals 22.72 kb on average. It's important to note that the average number of base pairs is not measured, so this calculation provides an estimate.
价格与库存信息目前不能提供
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一般描述
从雄性和雌性小牛的胸腺分离的高质量双链模板DNA。
应用
小牛胸腺DNA非常适合用于作为DNA聚合酶测定的底物、长片段的扩增、以及作为沉淀时的载体DNA。
特点和优势
•在37°C温育16小时后未检测到核酸酶活性
•高质量的模板DNA
•高质量的模板DNA
数量
1mg DNA约为20个 A260 单位。
制备说明
使用Aposhian和Kornberg的方法，从高度聚合的小牛胸腺DNA中进行制备，并用DNase进行切口。[3]
重悬
将DNA在分子生物级水中溶解过夜，终浓度为1mg/ml。
相关产品
产品编号
说明
价格
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
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Scientific reports, 10(1), 18334-18334 (2020-10-29)
Sex steroids, such as estrogens and androgens, are important regulators of the humoral immune response. Studies in female mice have demonstrated that alteration of circulating estrogen concentration regulates antibody-mediated immunity. As males have normally little endogenous estrogen, we hypothesized that
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PARP-1 synthesizes long poly(ADP-ribose) chains (PAR) at DNA damage sites to recruit DNA repair factors. Among proteins relocated on damaged DNA, the RNA-binding protein FUS is one of the most abundant, raising the issue about its involvement in DNA repair.
Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.
H V APOSHIAN et al.
The Journal of biological chemistry, 237, 519-525 (1962-02-01)
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Could you provide the number of base pairs for the D4522 product? For example, is it around 20kb?
1 answer-
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What is the solution stability of Product D4522, Deoxyribonucleic acid?
1 answer-
Solutions of deoxyribonucleic acid have been stored successfully for several months at 4°C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations (25 μg/mL), DNA tends to absorb onto the surfaces of plastic tubes. It is not recommended to store deoxyribonucleic acid in alkaline solutions since it tends to degrade at pH greater than 8.0.
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Is BSA (bovine serum albumin) used during the method of preparation for Product D4522, Deoxyribonucleic acid?
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During the method of preparation, when deoxyribonucleic acid is nicked, BSA is added to the solution. This is followed by an extraction method to remove all exogenous protein. We do not, however, claim this deoxyribonucleic acid is 100% clean of all proteins (including BSA).
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What is the molecular weight of Product D4522, Deoxyribonucleic acid?
1 answer-
The molecular weight is reported to be between 10-15 million Daltons prior to activation (nicking).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Is Product D4522, Deoxyribonucleic acid, single or double stranded?
1 answer-
Even though it is activated or "nicked" DNA, we have found that it is primarily double stranded.
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