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Merck

D4764

Sigma-Aldrich

脱氧核糖核酸 来源于小牛胸腺

Genomic, unsheared

别名:

DNA 来源于小牛胸腺, 小牛胸腺DNA, 胸腺核酸

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
41106310
eCl@ss:
32160414
NACRES:
NA.52

等級

for molecular biology

形狀

lyophilized powder

分子量

>20 kb

儲存溫度

2-8°C

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一般說明

从雄性和雌性小牛的胸腺分离的高质量双链模板DNA。

特異性

42% GC;Tm = 87 °C.
%GC是指G/C碱基对的百分比。Tm是热变性曲线中点处的温度。

應用

小牛胸腺DNA非常适合用作DNA聚合酶检测的底物、用于扩增非常长的片段以及用作DNA沉淀的载体。它被用作DNA荧光定量的标准品。它被用作参考物质,用于检测软骨组织消化样品的DNA含量。
小牛胸腺DNA非常适合用于作为DNA聚合酶测定的底物、长片段的扩增、以及作为沉淀时的载体DNA。

特點和優勢

•在37°C温育16小时后未检测到核酸酶活性
•高质量的模板DNA

準備報告

阴离子交换色谱纯化,用含1mM氯化钠、1mM EDTA和1mM Trizma HCl的溶液(pH 7.5)透析,并以约2 A260 单位/mL的浓度冻干。约20 260单位/mg DNA。

重構

将DNA溶解在分子生物级水中并轻轻摇动过夜,使终浓度为1mg/ml。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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