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Merck
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主要文件

CMC0015

Sigma-Aldrich

BL21(DE3)pLysE化学感受态细胞

Escherichia coli, rod shaped

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About This Item

UNSPSC代码:
41106202
NACRES:
NA.85

产品名称

BL21(DE3)pLysE化学感受态细胞, for protein expression

生物来源

Escherichia coli

等级

Molecular Biology
for molecular biology

生长模式

adherent or suspension

形态学

rod shaped

技术

microbiological culture: suitable

细胞转化

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

运输

dry ice

储存温度

−70°C

一般描述

The BL21(DE3) pLysE Chemically Competent Cells produce T7 lysozyme to repress low-level transcription from the T7 promoter prior to IPTG induction, helping to stabilize inserts coding for deleterious products.
These cells have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

特点和优势

  • a transformation efficiency of >1 × 107 cfu/μg.
  • economical prices
  • offering superior value for everyday protein expression work.

组分

  • BL21(DE3) pLysE chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


储存分类代码

10 - Combustible liquids


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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相关内容

BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

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