CTX TNA2

98102213, rat brain, Neuronal (astrocyte)

• 分類程式碼代碼: 41106514

属性

• product name: CTX TNA2, 98102213
• 生物源: rat brain
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Neuronal (astrocyte)
• 產品: Alpha 2 macroglobulin
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat Astrocyte, Transfected

細胞系描述

Established by transfecting cultures of primary astrocytes from brain frontal cortex tissue of 1 day old sprague-dawley rats with a DNA construct containing the oncogenic early region of SV40. Transcriptional control was effected using the human GFAP promoter (pGFA-SV-TE) and the murine phosphoglycerate kinase promoter (pPGK-neo). Cloning was achieved using G418. The cells are phenotypically similar to type 1 astrocytes. CTX TNA2 displays 20% positive staining for glial fibrillary acid protein (GFAP) and a β alanine inhibitable high affinity uptake mechanism for gamma amino butyric acid (GABA). α-2-macroglobulin production is similar to that found in primary astrocytes, but transferrin production is reduced. The cell line has been shown not to produce proenkephalin A, galactoceebroside or to express the 04 or A2B5 epitopes characteristic of type 2 astrocytes. Immunostaining has shown the SV40 T antigen to be present in over 95% of cells.

應用

Studies of the development and biochemistry of the central nervous system.

培養基

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Subculture before flasks become confluent or cells will detach in sheets reducing the effectiveness of trypsin

其他說明

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