MFM-223

98050130, human breast, Epithelial-like

• 别名: MFM223 Cells
• UNSPSC代码: 41106514

属性

• 产品名称: MFM-223, 98050130
• 生物来源: human breast
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Epithelial-like
• 产品: Not specified
• 受体: Androgen receptor
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human caucasian mammary carcinoma

细胞系描述

The mammary carcinoma cell line MFM-223 was obtained from the pleural effusion of a postmenopausal breast cancer patient. The patient had not received any prior treatment and the tumour was classified as a widespread ductal mammary carcinoma with the histological grade of III. The cell line is characterised by high androgen, (approximately 100,000 binding sites per cell) and low oestrogen and progesterone receptor levels. Proliferation of cells was inhibited by addition of the androgen dihydrotestosterone at concentrations above 0.01 nM. MFM-223 have a characteristic pattern of isoenzyme polymorphism and of karyotype alterations revealing marker chromosomes and homogenously staining regions. Most cells have been near diploid with 10% displaying near tetraploidy. The cells have been tested to be free of Hepatitis B and C viruses and HIV. MFM-223 is tumourigenic in nude mice.

应用

Androgen receptor studies, study of mammary carcinoma

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,12
D16S539: 11
D5S818: 11,12
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 17

培养基

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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