LNCap Clone FGC Cell Line human

89110211, human prostate (carcinoma), Epithelial-like

• 别名: LNCaP-FGC Cells
• 分類程式碼代碼: 41106514

属性

• product name: LNCap Clone FGC Cell Line human, from human prostate(carcinoma), 89110211
• 生物源: human prostate (carcinoma)
• 增長模式: Adherent
• 染色體組型: Pseudodiploid male; seven marker chromosomes, modal number 46, range 33 to 91
• 形態學: Epithelial-like
• 產品: Prostate acid phosphatase, prostate specific antigen
• 受體: Androgen, estrogen
• 技術: cell culture | mammalian: suitable
• 相關疾病: metastasis
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian prostate carcinoma

細胞系描述

Derived from a metastasis at the left supraclavicular lymph node of a 50 year old patient with a confirmed diagnosis of metastatic prostate carcinoma. Growth and acid phosphatase production is affected by 5-alpha-dihydrotestosterone. They do not form a uniform monolayer and attach only lightly to the substrate. When shipped, cells detach from flask and can either be incubated 24-48 hours to allow attachment or be collected by centrifugation (150xg, 15 minutes) and reseeded.

應用

LNCap Clone FGC Cell Line has been used:

• to study the effect of sex steroid hormones in regulating the expression of oligoadenylate synthetase 1 (OAS1)
• to study the role of curcumin and resveratrol in redox control and ability to promote apoptosis
• to investigate low molecular weight thiols present in the deproteinized extract of the cell line after derivatization

培養基

RPMI 1640 + 2mM Glutamine + 1.0 mM sodium pyruvate + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.i.e. seeding at 1-3 x 10,000 cells/cm² Cells grow slowly in clusters and can be disaggregated by repeated pipetting. After subculture cells may take up to 48 hours to reattach. Do not disturb during this period. Medium is rapidly acidified, therefore change twice per week.

其他說明

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