C2-Rev 7

94101907, rat liver, Epithelial

• UNSPSC代码: 41106514

属性

• 产品名称: C2-Rev 7, 94101907
• 生物来源: rat liver
• 生长模式: Adherent
• 核型: Hyperdiploid, 51 chromosomes
• 形态学: Epithelial
• 产品: Albumin and hepatocyte markers
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Rat hepatoma [HGPRT-]

细胞系描述

C2-Rev 7 is a revertant of the C2 variants, selected by virtue of its capacity to grow in glucose free medium (containing dialysed serum) where survival requires activity of the liver-specific gluconeogenic pathway. Expression of the entire set of liver specific functions is restored. Albumin and hepatocyte markers present.

应用

Gene expression studies.

培养基

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

传代培养常规

Split semi-confluent cultures (70%) 1:10 i.e. seeding at 9x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 15x1,000,000 cells/10cm² dish.

其他说明

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