Huh-7D 12 Cell Line human

1042712, human liver, Epithelial

• 别名: Huh7 Cells, JTC-39 Cells, Japanese Tissue Culture-39 Cells
• 分類程式碼代碼: 41106514

属性

• product name: Huh-7D 12 Cell Line human, 01042712
• 生物源: human liver
• 增長模式: Adherent
• 染色體組型: That of original Huh-7
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 儲存溫度: −196°C

说明

一般說明

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised. Huh-7D is a derivative of Huh-7 cells, which favor hepatitis C virus replication. Huh-7 cells are superior hepatic cell models compared to HepG2 cells for apolipoprotein based metabolic studies.

細胞系來源

Human hepatocellular carcinoma

細胞系描述

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised.

應用

Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV.

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16,18

培養基

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 C. Media change twice a week

其他說明

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