BRL 3A

85111503, rat liver, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: BRL 3A, 85111503
• 生物源: rat liver
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: MSA factors
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Buffalo Rat liver

細胞系描述

Originally isolated by primary cloning. Serum-free medium conditioned by the cells produces a family of polypeptides termed multiplication stimulating activity (MSA).

應用

Growth factor studies

培養基

Coon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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