Fao Cell Line from rat

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89042701, rat liver, Hepatocyte

• UNSPSC代码: 41106514

属性

• 生物来源: rat liver
• 包装: tube of 5 μg 89042701-DNA-5UG; pkg of vial of cells 89042701-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Hepatocyte
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Rat hepatoma

细胞系描述

A differentiated cell line derived from H4-11-E-C3 (Sigma catalogue no. 85061112). A versatile tool for studying regulation of mono-oxygenase activities and analysing their role in the activation of xenobiotics e.g. carcinogens, drugs and pesticides.

应用

Differentiation and enzymatic studies

培养基

Coon′s modified Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) or kaign′s modified Ham′s F12 + 45mg/l asorbic acid +18mg/1 inositol + 10% FBS +2mM Glutamine

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂ 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.