NMuMG

94081121, mouse breast, Epithelial

• UNSPSC代码: 41106514

属性

• 产品名称: NMuMG, 94081121
• 生物来源: mouse breast
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse mammary gland, normal epithelial

细胞系描述

Initiated from the mammary gland tissue of an adult NAMRU mouse and one of a series of epithelial cell lines derived from normal glandular tissue of mice. The cells exhibit many ultrastructural features of secretory epithelium as well as desmosomes or intermediate junctions, microvilli and tonofibrils. Hemicytes or domes develop when dense monolayers are maintained for several weeks without sub-cultivation. The cells produce benign cystadenomas when inoculated into isogenic mice and are negative for type C oncornaviruses.

应用

NMuMG has been used to compare the actions of insulin like growth factor binding protein 5 (IGFBP-5) with transforming growth factor β 1 (TGFβ1) on epithelial cells. It has also been used to measure the influence of phorbol 12-myristate 13-acetate (PMA) and estradiol on bone morphogenetic protein receptor type 2 (BMPR2) gene expression.

培养基

DMEM + 2mM Glutamine + 10ug/ml Insulin + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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