Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.
應用
DNA transfection studies
NIH 3T3 Cell Line has been used to:
study calcium-mediated actin reset (CaAR) in response to physiological changes[1]
study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation[2]
determine the viral titer of the retroviral construct -ST40hTNFα[3]
培養基
DMEM + 2mM Glutamine + 10% Calf Serum (CS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.
其他說明
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历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
