NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93061524, mouse embryo, Fibroblast
Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.
应用
DNA transfection studies
NIH 3T3 Cell Line has been used to:
study calcium-mediated actin reset (CaAR) in response to physiological changes[1]
study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation[2]
determine the viral titer of the retroviral construct -ST40hTNFα[3]
培养基
DMEM + 2mM Glutamine + 10% Calf Serum (CS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.
DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
