Neuro 2a Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89121404, mouse nerve, Neuronal/amoeboid-like

• 别名: N2a Cells, Nb2a Cells, Neuro2a Cells
• UNSPSC代码: 41106514

属性

• 生物来源: mouse nerve
• 描述: Mouse Albino neuroblastoma
• 包装: tube of 5 μg 89121404-DNA-5UG; pkg of vial of cells 89121404-1VL
• 生长模式: Adherent
• 核型: 2n = 40
• 形态学: Neuronal/amoeboid-like
• 产品: Microtubular protein
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse Albino neuroblastoma

细胞系描述

Derived from a spontaneous tumour in an albino strain A mouse. Cells produce microtubular protein which is believed to play a role in the contractile system giving axoplasmic flow in nerve cells.

应用

Neuro 2a cell line has been used as a model to study the potential toxicological effects of copper oxide nanoparticles accumulation in the brain. It has also been used to determine the sensitivity of the cell line to saxitoxin.[1][2]

Tumourigenicity studies, microtubular protein synthesis, vinblastine precipitation, GTP binding kinetics

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 4x10,000 cells cm²; 5% CO₂; 37°C. After resuscitation cells can take up to 6 days before a monolayer forms. These cells only adhere to the culture flask very lightly, care should be taken when subculturing. There is no need to wash the cells with PBS or trypsin, merely pour off half of the spent medium carefully, give the flask a gentle knock and resuspend the cells in fresh medium using a pipette to gently disperse any clumps of cells to finally reseed at 1:2. On arrival, growing cultures should be spun out and re-seeded in fresh media.

其他说明

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