M3 Clone M-3

93120827, mouse skin, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: M3 Clone M-3, 93120827
• 生物源: mouse skin
• 增長模式: Adherent
• 染色體組型: Modal no. 83; hypertetraploid
• 形態學: Epithelial
• 產品: Melanin
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse Cloudman S 91 melanoma

細胞系描述

Melanin producing cell line, derived from a Cloudman S 91 melanoma induced in a F1 (CxDBA) male mouse. The line is tumourigenic in mice and susceptible to a variety of viruses including herpes simplex, vaccinia, pseudorabies and vesicular stomatitis virus (Indiana virus).

應用

Tumourigenicity studies

培養基

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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