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Key Documents

P3U1

85011417, mouse skin, Lymphoblast

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About This Item

分類程式碼代碼:
41106514

product name

P3U1, 85011417

生物源

mouse skin

增長模式

Suspension

染色體組型

Not specified

形態學

Lymphoblast

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Mouse myeloma

細胞系描述

Derivative of P3X63Ag8, known as the Kearney line, the cell line is a non secretor. HGPRT- and HAT sensitive. It is useful for fusion with spleen cells to produce hybridomas or for hybridisation studies with other myelomas or lymphomas.

應用

Fusion partner, hybridisation and tumourigenicity studies

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Rapid cell death occurs when cell concentration exceeds 1,000,000 cells/ml.

其他說明

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Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

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