RAW 264.7 Cell Line murine

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. 91062702, mouse blood, Macrophage

• 别名: RAW264 7 Cells, RAW264.7 Cells
• UNSPSC代码: 41106514

属性

• 生物来源: mouse blood
• 包装: tube of 5 μg 91062702-DNA-5UG; vial of cells 91062702-1VL
• 生长模式: Semi-adherent
• 核型: Not specified
• 形态学: Macrophage
• 产品: Lysozyme
• 受体: Immunoglobulin, complement
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse monocyte macrophage

细胞系描述

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.

应用

Metabolic studies

RAW 264.7 cell line has been used to study the anti-inflammatory effects of pristimerin. It has also been used to test the internalization and intracellular survival of Listeria monocytogenes.

包装

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

培养基

DMEM + 2mM Glutamine + 10% FBS / FCS (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm²; 5% CO₂; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

其他说明

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