MEWO

93082609, human skin, Fibroblast

• UNSPSC代码: 41106514

属性

• 产品名称: MEWO, 93082609
• 生物来源: human skin
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Fibroblast
• 产品: Melanin
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human malignant melanoma

细胞系描述

Granular fibroblasts. Derived from a human melanoma by Prof. C Grose (1978). The cells support growth of varicella-zoster virus (VZV) isolates at 36°C although viral growth is optimal at 32°C.

应用

Virus studies

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA (0.5% trypsin in 0.53mM EDTA in modified Puck′s saline A); 5% CO₂; 37°C.

其他说明

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