RT4

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91091914, human bladder, Epithelial

• 别名: RT-4 Cells, RT4PCells
• UNSPSC代码: 41106514

属性

• 生物来源: human bladder
• 包装: tube of 5 μg 91091914-DNA-5UG; pkg of vial of cells 91091914-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human Caucasian bladder transitional-cell carcinoma

细胞系描述

Derived from explants of a recurring papillary tumour of the bladder.

应用

RT4 cell line has been used to study the effects of uropathogenic Escherichia coli on neutrophil adhesion via neutrophil adhesion assay.

Tumourigenicity studies

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8
D16S539: 9
D5S818: 11,12
D7S820: 9,12
THO1: 9,9.3
TPOX: 8,11
vWA: 14,17

培养基

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.