CCRF S-180 II

90011901, mouse unknown/unspecified, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: CCRF S-180 II, 90011901
• 生物源: mouse unknown/unspecified
• 增長模式: Adherent
• 染色體組型: 2n = 40
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse CFW sarcoma 180

細胞系描述

Established from an S-180 sarcoma maintained in adult CFW mice. The cells have been used extensively in chemotherapy screening tests.

應用

Chemotherapy screening tests

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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