MM537

12070509, human skin, Epithelial

• 别名: MM 537
• UNSPSC代码: 41106514

属性

• 产品名称: MM537, 12070509
• 生物来源: human skin
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Epithelial
• 受体: Not specified
• 相关疾病: metastasis

说明

细胞系来源

Human melanoma; from metastatic site - lymph node

细胞系描述

mM537 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1356.

DNA图谱分析

Not specified

培养基

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + 10% FBS.

传代培养常规

Optimal split ratio 1:4 (minimum seeding density 2.7 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 1-2 min. MM537 required growth medium to be changed 3 times each week. The cells are sensitive to trypsin so exposure time to trypsin should be kept to a minimum. The cells take 2-3 days to recover from trypsinisation.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.