TKE2

11033107, mouse cornea. Epithelial cells isolated from basal limbal layer.

• 别名: TKE, TKE-2
• 分類程式碼代碼: 41106514

属性

• product name: TKE2, from mouse(CD-1), female eye, 11033107
• 生物源: eye; mouse (CD-1)
• 性別: female
• 增長模式: Adherent
• 染色體組型: Normal karyotype of the female mouse CD1 strain
• 形態學: Epithelial; small and regular cobblestone-like shape
• 產品: Stains positive for nuclear p63 expression and K14 keratin.
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice

说明

細胞系來源

Mouse cornea epithelial progenitor cells

細胞系描述

TKE2 is the first cell line of mouse corneal epithelial progenitor cells isolated from the basal layer of the limbal epithelium.TKE2 has applications in the cell biology of corneal epithelial cells, toxicity, and differentiation. TKE2 can be induced to differentiate into stratified epithelium on increasing the calcium concentration and addition of foetal bovine serum.

DNA分析

Not specified

培養基

Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine.

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 2 x 10² - 2 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

其他說明

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