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Key Documents

WERI

6070602, human eye, Grape like clusters of round cells

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About This Item

分類程式碼代碼:
41106514

product name

WERI, 06070602

生物源

human eye

增長模式

Semi-adherent

染色體組型

Not specified

形態學

Grape like clusters of round cells

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Human Retinoblastoma

細胞系描述

Human retinoblastoma cell line derived from a female Caucasian. The cells are semi-adherent, some neuronal differentiation can occur when subculturing. Tumourigenic in rabbits.

培養基

Iscove′s Modified DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS) + 10ug/ml Insulin + 55uM 2-Mercaptoethanol

例行更新培養

Split subconfluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-3 x100,000 cells/ml; 5% CO2; 37°C. Saturation density: 2 x 1000,000 cells/ml. Cells are semi-adherent. Scrape cells from surface; do not use trypsin.

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