This product is not sterile as received. Collagen solutions may be sterilized by dialysis in a 0.25% acetic acid and 0.5% chloroform solution. We do not recommend sterilizing the collagen solution by membrane filtration as we have found substantial protein loss by this method. Coating of tissue culture plastic dishes may be performed by air-drying. Dried coated dishes can be sterilized by exposure to UV light in a sterile culture hood or by rinsing with 70% ethanol. Detailed information can be found in the link below:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/collagen-product-protocols
推荐产品
产品名称
胶原蛋白 来源于鸡胸软骨, Type II (Miller), powder, BioReagent, suitable for cell culture
生物来源
chicken (Sternal cartilage)
质量水平
类型
Type II (Miller)
产品线
BioReagent
表单
powder
包装
glass bottle of 100 mg
poly bottle of 25 mg
glass bottle of 5 mg
浓度
60-80% (biuret)
技术
cell culture | mammalian: suitable
表面覆盖度
6‑10 μg/cm2
溶解性
acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
UniProt登记号
结合特异性
Peptide Source: Fibrinogen
运输
wet ice
储存温度
2-8°C
基因信息
chicken ... COL2A1(395069)
相关类别
一般描述
胶原蛋白由三个多肽链组成。[1]2型胶原蛋白广泛存在于透明软骨中。它是由三个α1[2]链组成的三同聚体。[1]2型胶原蛋白也主要存在于椎间盘髓核和眼玻璃体中。[2]从鸡胸骨软骨中提取的蛋白多糖与九型胶原蛋白结构相似。[3]
胶原蛋白被分为许多种结构和遗传性不同的类型。我们使用Bornstein和Traub提出的命名法。不要混淆Sigma分类标识和公认的胶原蛋白分类类型。
应用
生化/生理作用
胶原蛋白在体内会代谢降解，释放来自氨基端的N-端肽。还会释放可能来自羧基端的C-端肽。N-端肽进入尿液，在诊断检验中可用来筛查骨质疏松症。
特点和优势
该胶原蛋白已通过哺乳动物细胞培养检验，确认产品内毒素含量低。
其他说明
关节软骨的主要成分。
制备说明
通过Trentham, D.E.等人的改良方法制备。
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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Is this product sterilized? If not, how do we sterilize it? Thank you!
1 answer-
Helpful?
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May I please know the exact molecular weight of the protein ?
1 answer-
The product C9301 gives 1 major band at approximately 150 KDa in the SDS-PAGE test.
Helpful?
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What is the recommended method for storing stock solutions of item C9301, Collagen from chicken sternal cartilage after reconstitution?
1 answer-
Following reconstitution, store the solution in the refrigerator at 4°C. Stock solutions are stable for up to 6 months at 4°C. It is important to avoid freeze/thaw cycles of the solutions.
Helpful?
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