跳转至内容
Merck

C7698

Sigma-Aldrich

环己酰亚胺,大包装

from microbial, ≥94% (TLC)

别名:

3- [2-(3,5-二甲基-2-氧代环己基)-2-羟乙基]戊二酰亚胺, 放线菌酮, 纳拉霉素A

登录查看公司和协议定价


About This Item

经验公式(希尔记法):
C15H23NO4
CAS号:
分子量:
281.35
Beilstein:
88868
EC 号:
MDL编号:
UNSPSC代码:
12352111
PubChem化学物质编号:
NACRES:
NA.77
价格与库存信息目前不能提供

生物来源

microbial

方案

≥94% (TLC)

表单

powder

颜色

white to off-white

溶解性

ethanol: soluble, clear to hazy

抗生素抗菌谱

fungi
yeast

作用机制

protein synthesis | interferes

储存温度

2-8°C

SMILES字符串

[H][C@]1(C[C@@H](C)C[C@H](C)C1=O)[C@H](O)CC2CC(=O)NC(=O)C2

InChI

1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1

InChI key

YPHMISFOHDHNIV-FSZOTQKASA-N

基因信息

正在寻找类似产品? 访问 产品对比指南

一般描述

环己酰亚胺,也称放线菌酮,是一种戊二酰亚胺娄抗生素,通常来源于灰色链霉菌。它可用作真核细胞中蛋白质生物合成的强效抑制剂,其机理是破坏翻译中的转运步骤,从而有效阻止翻译延伸(translational elongation)。在微生物学领域,作为酵母和真菌抗性菌株的选择剂它起到关键作用,在受控实验环境中非常重要。

在细胞生物学和生物化学研究领域,环己酰亚胺体现出与诱导细胞凋亡有关的双重特性,即根据细胞类型不同诱导或抑制细胞凋亡。快速且可逆的作用令其成为研究细胞过程和测定蛋白质半衰期的理想选择。环己酰亚胺也在生物医学研究中得到广泛应用,它抑制体外(生物体外)研究的真核细胞中的蛋白质合成,去除环己酰亚胺则可快速逆转其作用。这一特性使环己酰亚胺成为细胞生物学、生物医学和生物化学研究的最佳选择,为实验带来了精准的控制和多功能性。

应用

  • 在酵母菌株中,环己酰亚胺已被用作环己酰亚胺追踪实验中的蛋白质合成抑制剂。[1]
  • 它已被用于抑制哺乳动物细胞中的翻译。[2][3]
  • 它已被用于抑制真菌生长。[4]

生化/生理作用

作用机制:真核生物中的翻译抑制导致细胞生长停滞和细胞死亡。CHX被广泛用于抗CHX的酵母和真菌菌株的选择、蛋白质合成的受控抑制以检测短寿命蛋白质和蛋白质表达的超诱导作用、以及诱导凋亡或促进通过死亡受体诱导的凋亡。

活性谱:具有抑制酵母和真菌如念珠菌、曲霉、酵母菌和青霉的活性

特点和优势

  • 适用于多种研究应用的优质抗生素
  • 常用于细胞生物学和生物化学研究

储存及稳定性

密闭。干燥。于通风良好处保存。上锁保存,或保存在仅对有资格或授权人员
开放的区域。

其他说明

保存于密闭容器内,置于干燥通风处。置于干燥处保存。
如需了解生化试剂系列的更多信息,请填写此表

警示用语:

Danger

危险分类

Acute Tox. 2 Oral - Aquatic Chronic 2 - Muta. 2 - Repr. 1B

储存分类代码

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

其他客户在看

Quality control of a transcriptional regulator by SUMO-targeted degradation.
Wang Z and Prelich G
Molecular and Cellular Biology, 29, 1694-1694 (2009)
Potential pathogenic bacteria in metalworking fluids and aerosols from a machining facility.
Perkins SD and Angenent LT
FEMS Microbiology Ecology, 74, 643-643 (2010)
Nonsense-mediated mRNA decay (NMD) blockage promotes nonsense mRNA stabilization in protein 4.1R deficient cells carrying the 4.1R Coimbra variant of hereditary elliptocytosis.
Moriniere M et al.
Blood Cells, Molecules and Diseases, 45, 284-284 (2010)
Zhen Zhang et al.
Frontiers in endocrinology, 5, 206-206 (2014-12-19)
O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification involving an attachment of a single β-N-acetylglucosamine moiety to serine or threonine residues in nuclear and cytoplasmic proteins. Cellular O-GlcNAc levels are regulated by two enzymes: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), which
Ming-Yuh Shiau et al.
Scientific reports, 9(1), 11974-11974 (2019-08-21)
Accumulating evidence indicates that inflammation participates in the pathophysiological progress from insulin resistance, obesity, metabolic abnormalities, and type 2 diabetes mellitus. Our previous study reveals that interleukin-4 (IL-4) inhibits adipogenesis and promotes lipolysis to decrease lipid deposits by enhancing the

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

Reviews

No rating value

Active Filters

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门