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生物源
mouse
品質等級
共軛
CY3 conjugate
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
AC-40, monoclonal
形狀
buffered aqueous solution
分子量
antigen 42 kDa
物種活性
human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat
技術
direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts
同型
IgG2a
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
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一般說明
该抗体可识别在所有肌动蛋白亚型中均保守的C末端表位。它能够特异性标记各种组织和物种中的肌动蛋白。 被抗体识别的表位能够耐受甲醛固定和石蜡包埋。锌-福尔马林、B5、乙醇、methacarn、Brunnel's或Bouin's溶液也可用作固定液。
Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin has six isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (α-skeletal, α-cardiac and α-smooth muscle), one β-actin (β-nonmuscle) and two γ-actins (γ-smooth muscle and γ-nonmuscle).
免疫原
与多抗原肽（MAP）骨架连接的合成肌动蛋白C末端肽
應用
已使用肌动蛋白单克隆抗体作为一抗，对MDCK细胞裂解物进行蛋白质免疫印迹分析。
肌动蛋白单克隆抗体被用于Cos-7细胞裂解物的蛋白质免疫印迹分析，以确保等量蛋白质上样。
Monoclonal Anti-Actin-Cy3 antibody has been used in immunofluorescent staining.
生化／生理作用
Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
其他說明
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
法律資訊
Distributed under license from Amersham Biosciences Limited.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Erin Faught et al.
Scientific reports, 8(1), 18081-18081 (2018-12-29)
The mineralocorticoid receptor (MR) in mammals mediates the effects of aldosterone in regulating fluid balance and potassium homeostasis. While MR signalling is essential for survival in mammals, there is no evidence that MR has any physiological role in ray-finned fish.
Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.
Gunning P, et al.
Molecular and Cellular Biology, 3(5), 787-795 (1983)
A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
Skalli O, et al.
The Journal of Cell Biology, 103(6), 2787-2796 (1986)
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