F3046

单克隆抗肌动蛋白抗体

clone AC-40, purified immunoglobulin, buffered aqueous solution

• 别名: Monoclonal Anti-Actin
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 200
• 共軛: FITC conjugate
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: AC-40, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 42 kDa
• 物種活性: human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat
• 技術: direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts
• 同型: IgG2a
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ACTA1(58) , ACTA1(58) , ACTA1(58) , ACTA2(59) , ACTA2(59) , ACTA2(59) , ACTB(60) , ACTB(60) , ACTB(60) , ACTC1(70) , ACTC1(70) , ACTC1(70) , ACTG1(71) , ACTG1(71) , ACTG1(71) , ACTG2(72) , ACTG2(72) , ACTG2(72) ; mouse ... Acta1(11459) , Acta1(11459) , Acta1(11459) , Acta2(11475) , Acta2(11475) , Acta2(11475) , Actb(11461) , Actb(11461) , Actb(11461) , Actc1(11464) , Actc1(11464) , Actc1(11464) , Actg1(11465) , Actg1(11465) , Actg1(11465) , Actg2(11468) , Actg2(11468) , Actg2(11468) ; rat ... Acta1(29437) , Acta1(29437) , Acta1(29437) , Acta2(81633) , Acta2(81633) , Acta2(81633) , Actb(81822) , Actb(81822) , Actb(81822) , Actc1(29275) , Actc1(29275) , Actc1(29275) , Actg1(287876) , Actg1(287876) , Actg1(287876) , Actg2(25365) , Actg2(25365) , Actg2(25365)

说明

一般說明

该抗体可识别在所有肌动蛋白亚型中均保守的C末端表位。它能够特异性标记各种组织和物种中的肌动蛋白。 被抗体识别的表位能够耐受甲醛固定和石蜡包埋。锌-福尔马林、B5、乙醇、methacarn、Brunnel's或Bouin's溶液也可用作固定液。

Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. It has one N-terminal region which appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

免疫原

与多抗原肽（MAP）骨架连接的合成肌动蛋白C末端肽

應用

已使用肌动蛋白单克隆抗体作为一抗，对MDCK细胞裂解物进行蛋白质免疫印迹分析。

肌动蛋白单克隆抗体被用于Cos-7细胞裂解物的蛋白质免疫印迹分析，以确保等量蛋白质上样。

Actin–FITC antibody is suitable for direct immunofluorescence at a dilution of 1:75, using cultured human or chicken fibroblasts.

生化／生理作用

Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. It is associated with myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. In mammals and birds, actin is expressed as six different isoforms. It has one N-terminal region which appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

其他說明

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable