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Merck

A4418

Sigma-Aldrich

硫酸铵

Ammonium sulfate
1 of 2 reviewers received a sample product or took part in a promotion

for molecular biology, ≥99.0%

别名:

硫铵石

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About This Item

线性分子式:
(NH4)2SO4
CAS号:
分子量:
132.14
EC號碼:
MDL號碼:
分類程式碼代碼:
12352314
eCl@ss:
38030408
PubChem物質ID:
NACRES:
NA.31
化驗:
≥99.0%
等級:
for molecular biology
形狀:
powder
溶解度:
H2O: 0.667 g/mL
价格与库存信息目前不能提供

等級

for molecular biology

品質等級

化驗

≥99.0%

形狀

powder

技術

affinity binding assay: suitable
protein purification: suitable

雜質

≤0.005% Insolubles

pH值

5.0-6 (25 °C, 132 g/L)

mp

>280 °C (dec.) (lit.)

溶解度

H2O: 0.667 g/mL

密度

1.77 g/mL at 25 °C (lit.)

負離子痕跡

chloride (Cl-): ≤5 ppm

正離子痕跡

Cu: ≤5 ppm
Fe: ≤5 ppm
Zn: ≤5 ppm
heavy metals (as Pb): ≤2 ppm

吸收

≤0.025 at 280 at 1 M
≤0.03 at 260 at 1 M

適合性

suitable for flow cytometry
suitable for molecular biology

應用

metabolomics
microbiology

異物活動

DNase, RNase, and protease, none detected

SMILES 字串

N.N.OS(O)(=O)=O

InChI

1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)

InChI 密鑰

BFNBIHQBYMNNAN-UHFFFAOYSA-N

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一般說明

硫酸铵是一种用途广泛的无机硫酸盐,在生化研究、分子生物学和细胞培养中具有至关重要的作用。它是纯化抗体和沉淀或分离蛋白质不可或缺的工具。[1]此外,它有助于核酸和蛋白质的晶体学分析,还可用作合成实验室培养基中的氮补充剂。[2] 我们的精品硫酸铵经过精心配制,不含 DNase、RNase 和蛋白酶,确保其适合尖端研究。

應用

硫酸铵有助于细胞蛋白的快速沉淀。 硫酸铵可用于蛋白质的沉淀或分级,或抗体的纯化。它也用于核酸和蛋白质的晶体学分析。硫酸铵已用于
  • 蛋白的′蓝银′ 胶体考马斯染液的制备。[3]
  • 沉淀培养上清液中的志贺样毒素(Shiga-like toxin)。[4]
  • 沉淀培养上清液中的病毒颗粒。 [5]

特點和優勢

  • 重金属(以铅计):≤2 ppm
  • 不含 RNase/DNase和蛋白酶。

其他說明

如需了解生化试剂系列的更多信息,请填写此表

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儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Asian Pacific Journal of Cancer Prevention, 23-10, 3247-3252 (2022)
Protein precipitation using ammonium sulfate
P Wingfield
Current Protocols in Protein Science / Editorial Board, John E. Coligan ... [Et Al.], 3 (2001)
Mohd Baasir Gaisawat et al.
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Clostridium (C.) difficile-infection (CDI), a nosocomial gastrointestinal disorder, is of growing concern due to its rapid rise in recent years. Antibiotic therapy of CDI is associated with disrupted metabolic function and altered gut microbiota. The use of probiotics as an
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A protocol for selective and site-specific enzymatic labeling of proteins is described. The method exploits the protein co-/post-translational modification known as myristoylation, the transfer of myristic acid (a 14-carbon saturated fatty acid) to an N-terminal glycine catalyzed by the enzyme

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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Reviews

1 of 2 reviewers received a sample product or took part in a promotion

Active Filters

  1. New Jersey, USA
    • Review 1
    • Vote 1
    5 out of 5 stars.

    works well

    I used this product to perform ammonium sulfate-mediated precipitation on cell culture media, and it has worked very well. I will keep buying this in future.

    Helpful?

  2. 1 Ratings-Only Review

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