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Merck
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主要文件

A3641

Sigma-Aldrich

去铁铁蛋白 来源于马脾脏

0.2 μm filtered

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About This Item

CAS号:
MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.32
价格与库存信息目前不能提供

质量水平

无菌性

0.2 μm filtered

表单

liquid

分子量

major subunit ML 19,889
minor subunit MH 22,200
native ~481.2 kDa (24 subunits, approx. 20 kDa each)

颜色

clear to slightly hazy, solution

储存温度

2-8°C

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应用

脱铁铁蛋白(来自马脾脏)已用于:
  • 测定其在相系统中的分配系数[1]
  • 溶于NaCl用于培养细胞的铁装载[2]
  • 在原位液体扫描透射电镜中用于生物学和纳米技术的界面成像[3]

生化/生理作用

这种缺铁铁蛋白的蛋白质外鞘广泛用于凝胶过滤柱和十二烷基硫酸钠(SDS)-聚丙烯酰胺凝胶电泳的校准。[4] 它在胰腺的β细胞中非常丰富,在那里充当抗氧化剂。[5]添加到培养的内皮细胞中后,去铁铁蛋白以剂量反应方式摄取,保护细胞免受氧化剂介导的细胞溶解。 [6]

外形

0.135 M氯化钠溶液。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

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Ferritin-iron increases killing of Chinese hamster ovary cells by X-irradiation
Nelson JM and Stevens RG
Cell proliferation, 25(6), 579-585 (1992)
Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy
Evans JE, et al.
Micron (Oxford, England : 1993), 43(11), 1085-1090 (2012)
Jorge H Melillo et al.
Scientific reports, 12(1), 16512-16512 (2022-10-04)
Some of the best nucleating agents in nature are ice-nucleating proteins, which boost ice growth better than any other material. They can induce immersion freezing of supercooled water only a few degrees below 0 °C. An open question is whether this
Hamish G Brown et al.
Communications biology, 5(1), 817-817 (2022-08-15)
Ice thickness is arguably one of the most important factors limiting the resolution of protein structures determined by cryo-electron microscopy (cryo-EM). The amorphous atomic structure of the ice that stabilizes and protects biological samples in cryo-EM grids also imprints some
Masahide Takehara et al.
Analytical biochemistry, 373(2), 322-329 (2007-12-07)
Two kinds of layer silicate powder, Micromica and chlorite, were used to aid protein crystallization by the addition to hanging drops. Using appropriate crystallization buffers, Micromica powder facilitated crystal growth speed for most proteins tested in this study. Furthermore, the

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