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生物源
goat
共軛
alkaline phosphatase conjugate
抗體表格
affinity isolated antibody
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous glycerol solution
物種活性
human
技術
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
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一般說明
人IgG是糖蛋白抗体,含有两条相等的轻链和一对相同的重链。 IgG具有四种不同亚型,范围从IgG1到IgG4。这些抗体可调节机体对过敏和病原性感染的免疫反应。IgG还与补体结合和自身免疫疾病有关。经测试证明,抗人IgM(γ-链特异性)-碱性磷酸酶抗体针对人IgA、IgG、IgM、本周蛋白κ和λ骨髓瘤蛋白具有特异性。
免疫原
纯化人IgG
應用
将从大肠杆菌( E. coli )培养物中收集的蛋白质上样至SDS-page凝胶进行蛋白质免疫印迹分析,使用浓度为1:10000的碱性磷酸酶标记山羊抗人IgG(链特异性)作为二抗。使用按1:5000稀释于0.25% BSA/PBS中的碱性磷酸酶标记山羊抗人IgG(γ链特异性)抗体,通过Elisa法检测人血清中的IgG。使用对硝基苯磷酸底物(1mg/ml,Sigma)进行显色。
抗人IgG(γ-链特异性)-碱性磷酸酶抗体适用于ELISA检测(稀释度为1:2500和1:10,000)。该抗体还能用于蛋白质免疫印迹 (1:30,000)和免疫组织化学分析(使用甲醛固定石蜡包埋切片时,稀释度为1:50)。
外觀
溶剂为0.05 M Tris缓冲液,pH 8.0,含1 mM MgCl2、10 mM甘氨酸、1%牛血清白蛋白、50%甘油和15 mM叠氮化钠
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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