I used it for an ELISA aimed at detect specific autoantibodies or total IgG in human plasma at 1:5000 and worked very well.
推荐产品
生物来源
goat
偶联物
peroxidase conjugate
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
种属反应性
human
技术
direct ELISA: 1:60,000
dot blot: 1:100,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
人IgG是糖蛋白抗体，含有两条相等的轻链和一对相同的重链。IgG具有四种不同亚型，范围从IgG1到IgG4。编码IgG基因簇的基因位于人14号染色体上。这些抗体可调节机体对过敏和病原性感染的免疫反应。IgG还与补体结合和自身免疫疾病有关。
通过免疫特异性纯化从山羊抗人IgG抗血清中获得亲和力分离的抗体，该过程可基本上去除所有山羊血清蛋白，包括免疫球蛋白（其不可与人IgG的Fc片段特异性结合）。将抗人IgG与过氧化物酶结合，以去除未结合的物质进行纯化。
通过ELISA确定抗人IgG（Fc特异性）-过氧化物酶的特异性和交叉反应性。当对人IgA、IgG（Fab和Fc片段）、IgM、Bence Jones kappa和λ骨髓瘤蛋白进行检测时，该结合物仅对人IgG（Fc片段）具有特异性。此外，结合物对小鼠或大鼠IgG没有反应性，并且与小鼠或大鼠样品产生较少的背景。
通过免疫特异性纯化从山羊抗人IgG抗血清中获得亲和力分离的抗体，该过程可基本上去除所有山羊血清蛋白，包括免疫球蛋白（其不可与人IgG的Fc片段特异性结合）。将抗人IgG与过氧化物酶结合，以去除未结合的物质进行纯化。
通过ELISA确定抗人IgG（Fc特异性）-过氧化物酶的特异性和交叉反应性。当对人IgA、IgG（Fab和Fc片段）、IgM、Bence Jones kappa和λ骨髓瘤蛋白进行检测时，该结合物仅对人IgG（Fc片段）具有特异性。此外，结合物对小鼠或大鼠IgG没有反应性，并且与小鼠或大鼠样品产生较少的背景。
特异性
通过ELISA确定抗人IgG（Fc特异性）-过氧化物酶的特异性。当对人IgA、IgG（Fab和Fc片段）、IgM、Bence Jones kappa和λ骨髓瘤蛋白进行检测时，该结合物仅对人IgG（Fc片段）具有特异性。通过ELISA确定抗体-缀合物的交叉反应性。结合物对小鼠或大鼠IgG没有反应性，并且与小鼠或大鼠样品产生较少的背景。
免疫原
IgG的Fc片段
应用
过氧化物酶标记山羊抗人IgG（Fc′特异性）抗体可用于通过ELISA（1：50000）法检测rifin蛋白处理后人血浆样品中的IgG活性水平。
过氧化物酶标记的山羊抗人IgG抗体适用于ELISA （1:60,000）、斑点杂交印迹（1:100,000）、免疫组织化学（1:150）和亲和层析色谱应用。
生化/生理作用
抗体可调节机体对过敏和病原性感染的免疫反应。IgG还与补体结合和自身免疫疾病有关。糖基化对于IgG功能至关重要。IgG亚类具有抵抗细菌和病毒感染的治疗应用潜力。[1]免疫球蛋白重链的基因易位与多发性骨髓瘤的发病机理有关。
外形
溶解在0.01M磷酸缓冲盐,pH7.4, 含有0.05%硫柳汞。
制备说明
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand.J. Immunol., 8, Suppl. 7, 7 (1978).
储存及稳定性
如需连续使用，可在2-8 °C保存至多一月。对于更长期的存储，可对溶液进行等分并冷冻保存。不建议反复冻融或存放在“无霜”冰柜中。如果长期储存时出现轻微浑浊，请在使用前通过离心澄清溶液。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
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Review the key factors that should figure in your decision to choose a secondary antibody. Learn about species, subclass, isotype, label, and more.
Review the key factors that should figure in your decision to choose a secondary antibody. Learn about species, subclass, isotype, label, and more.
Review the key factors that should figure in your decision to choose a secondary antibody. Learn about species, subclass, isotype, label, and more.
Review the key factors that should figure in your decision to choose a secondary antibody. Learn about species, subclass, isotype, label, and more.
实验方案
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
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