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Merck
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文件

A0293

Sigma-Aldrich

抗人 IgG（Fab 特异性）过氧化物酶 山羊抗

affinity isolated antibody

别名:

抗人Fab抗体, 抗人Fab抗体 - 抗人IgG（Fab特异）- 过氧化物酶抗体（山羊源）

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About This Item

MDL號碼:
MFCD00162434
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

共軛

peroxidase conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

物種活性

human

技術

direct ELISA: 1:40,000
dot blot: 1:100,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot (chemiluminescent): 1:100,000

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

相关类别

一般說明

人IgG是糖蛋白抗体，含有两条相等的轻链和一对相同的重链。 IgG具有四种不同亚型，范围从IgG1到IgG4。这些抗体可调节机体对过敏和病原性感染的免疫反应。IgG还与补体结合和自身免疫疾病有关。
通过免疫特异性纯化从抗人IgG抗血清中分离抗体，该过程可基本上去除所有山羊血清蛋白，包括免疫球蛋白（其不可与人IgG的Fab片段特异性结合）。将抗人IgG与过氧化物酶缀合，然后进一步纯化以除去未缀合的物质。

免疫原

人IgG的Fab片段

應用

山羊抗人IgG（Fab特异性）- 过氧化物酶抗体可用于直接ELISA（1：40,000）、斑点印迹和免疫组织化学（福尔马林固定，石蜡包埋切片；1：200）等应用。
山羊源抗人IgG（Fab特异性）-过氧化物酶抗体已用于以下应用：
  • 蛋白质印迹
  • 直接酶联免疫吸附测定(ELISA)（1:40,000稀释),以分析产生重组Fab片段的转基因酵母培养物。
  • 免疫组化
  • 斑点杂交
  • 免疫组化（福尔马林固定石蜡包埋切片，1:200）

在PBS中以1：10,000稀释抗人IgG抗体，并在室温下温育1小时。使用O-亚苯基-二胺底物溶液（Sigma）检测ELISA反应体系中的结合情况。

外觀

含0.05％MIT的0.01 M磷酸盐缓冲盐溶液(pH 7.4)

準備報告

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand.J. Immunol., 8, Suppl. 7, 7 (1978).

免責聲明

除非我们的产品目录或产品随附的其他公司文档中另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人或动物。

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象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H317

危險分類

Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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James Duehr et al.
mSphere, 3(1) (2018-02-13)
Recent reports in the scientific literature have suggested that anti-dengue virus (DENV) and anti-West Nile virus (WNV) immunity exacerbates Zika virus (ZIKV) pathogenesis in vitro and in vivo in mouse models. Large populations of immune individuals exist for a related
Hanieh Khalili et al.
Scientific reports, 6, 36905-36905 (2016-11-23)
Infliximab is an antibody that neutralizes TNF-α and is used principally by systemic administration to treat many inflammatory disorders. We prepared the antibody mimetic Fab-PEG-Fab (FpFinfliximab) for direct intravitreal injection to assess whether such formulations have biological activity and potential
Chen Li et al.
Acta pharmacologica Sinica, 32(3), 329-337 (2011-02-01)
To transform the human anti-rabies virus glycoprotein (anti-RABVG) single-chain variable fragment (scFv) into a Fab fragment and to analyze its immunological activity. The Fab gene was amplified using overlap PCR and inserted into the vector pComb3XSS. The recombinant vector was
Kristell Lebozec et al.
mAbs, 9(6), 945-958 (2017-06-10)
Glycoprotein VI is a platelet-specific collagen receptor critical for in vivo formation of arterial thrombosis. It is also considered as an attractive target for the development of anti-thrombotic drugs because blocking glycoprotein (GP)VI inhibits platelet aggregation without inducing detrimental effects
A novel approach of prophylaxis to HBV recurrence after liver transplantation
Pan T, et al.
Virology, 382(1), 1-9 (2008)
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