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Merck

N2125

Sigma-Aldrich

萘酚 AS-BI 磷酸盐

≥98% purity (HPLC), powder

别名:

7-溴-3-羟基-2-萘-o-茴香醚磷酸盐

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About This Item

经验公式(希尔记法):
C18H15BrNO6P
CAS号:
分子量:
452.19
Beilstein:
6668526
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47

产品名称

萘酚 AS-BI 磷酸盐, ≥98% (HPLC), Technical grade

质量水平

方案

≥98% (HPLC)

表单

powder

技术

microbe id | staining: suitable

颜色

white to light yellow

溶解性

methanol: 50 mg/mL

应用

diagnostic assay manufacturing
hematology
histology

储存温度

−20°C

SMILES字符串

COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP(O)(O)=O

InChI

1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)

InChI key

HUXIAXQSTATULQ-UHFFFAOYSA-N

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一般描述

色酚AS-BI(也称为7-溴代-3- 羟基-2-萘酚-o-茴香胺)是一种取代萘酚。

应用

萘酚AS-BI磷酸可用于:
  • 抗酒石酸酸性磷酸酶(TRAP)染色,以检测破骨细胞活性
  • Tris-HCl染色细胞的组成部分,以测定碱性磷酸酶(ALP)的活性
  • 进行碱性磷酸酶反应
酸性和碱性磷酸酶组织化学显示的底物。 酸性和碱性磷酸酶荧光测定法的底物。石蜡包埋组织中酸性磷酸酶的证明。单个活细胞中酸性磷酸酶的细胞荧光测定法。

生化/生理作用

色酚AS-BI可用作测定碱性和酸性磷酸酶的底物。也可用于测定单细胞酸性磷酸酶的活性。

底物

酸性和碱性磷酸酶组织化学显示的底物。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Z Szegedi et al.
Pathology oncology research : POR, 4(3), 217-223 (1998-10-08)
The aim of the study was to work out a technique for the detection of acid phosphatase enzyme activity by confocal laser-scanning microscope using the histochemical acid phosphatase detection method (after Barka and Anderson 1962, modified by Bowen and Lewis
W M Frederiks et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 35(2), 175-180 (1987-02-01)
Acid phosphatase activity has been demonstrated in rat liver with the semipermeable membrane technique using naphthol AS-BI phosphate as substrate and hexazotized pararosaniline (HPRA) as simultaneous coupling agent. With this method the final reaction product (FRP) appeared in rat liver
The presence of a low molecular weight acid phosphatase in liver tissue that cannot be demonstrated with the histochemical substrate naphthol AS-BI phosphate.
A E Meijer et al.
Histochemistry, 67(1), 23-29 (1980-01-01)
M Kimura et al.
Histochemistry, 82(6), 519-523 (1985-01-01)
A cytochemical study of acid phosphatase (AcP-ase) in the lateral prostate of the rat was performed to investigate whether AcP-ase in the secretory apparatus can be distinguished from AcP-ase in lysosomes and their related structures. Two types of AcP-ase were
Anthony J Janckila et al.
Clinica chimica acta; international journal of clinical chemistry, 347(1-2), 157-167 (2004-08-18)
Our purpose was to develop a specific immunoassay for tartrate-resistant acid phosphatase (TRACP) 5b using naphthol ASBI phosphate (N-ASBI-P) as a selective substrate for isoform 5b and heparin as a selective inhibitor of isoform 5a. Serum TRACP 5a and 5b

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