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Merck

B8647

Sigma-Aldrich

亮蓝R浓缩液

methanol solution

别名:

酸性蓝83, Coomassie 亮蓝R, 亮吲哚蓝蛋白 6B, 酸性蓝 83

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About This Item

经验公式(希尔记法):
C45H44N3NaO7S2
CAS号:
分子量:
825.97
顏色索引編號:
42660
Beilstein:
5718025
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47
价格与库存信息目前不能提供

产品名称

亮蓝R浓缩液, suitable for SDS-PAGE, methanol solution

形狀

methanol solution

品質等級

顏色

dark blue

適合性

suitable for SDS-PAGE

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

SMILES 字串

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI 密鑰

NKLPQNGYXWVELD-UHFFFAOYSA-M

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應用

亮蓝R浓缩液是一种甲醇基染液,专为聚丙烯酰胺中的蛋白质的染色而设计,而它也可对琼脂糖凝胶进行染色。染液含有甲醇和乙酸,因此凝胶在染色前不需要固定。
亮蓝R浓缩液已用于非变性梯度凝胶电泳、等电聚焦和SDS-PAGE(十二烷基硫酸钠聚丙烯酰胺凝胶电泳)后的蛋白条带检测。[1][2][3]
电泳后检测蛋白条带。

重構

用去离子水稀释至最终体积为1升后,溶液中将含有0.25% (w/v)亮蓝R、40% (v/v) 甲醇和7% (v/v)乙酸。

法律資訊

相關產品

产品编号
说明
价格

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 1

標靶器官

Eyes,Central nervous system

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

57.2 °F - closed cup

閃點(°C)

14 °C - closed cup

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Crystallization of soluble proteins in vapor diffusion for x-ray crystallography.
Benvenuti M and Mangani S
Nature Protocols, 2, 1633-1651 (2007)
Overexpression of human apolipoprotein AI in transgenic rats and the hyperlipoproteinemia associated with experimental nephrosis.
Burkey BF, et al.
Journal of Lipid Research, 36.7, 1463-1473 (1995)
Katharina Stock et al.
Cancer cell international, 19, 77-77 (2019-04-13)
The nucleation-promoting factor cortactin is expressed and promotes tumor progression and metastasis in various cancers. However, little is known about the biological role of cortactin in the progression of pancreatic ductal adenocarcinoma (PDAC). Cortactin and phosphorylated cortactin (Y421) were investigated
The effect of ethanol or hepatotoxin exposure on rat transferrin desialylation.
Lof K, et al.
Alcohol and Alcoholism (Oxford, Oxfordshire), 31, 445-451 (1996)
G Houen et al.
Electrophoresis, 18(5), 701-705 (1997-05-01)
A method for staining proteins on polyvinylidene difluoride membranes without using organic solvent is described. The method uses preblocking of the membrane with either Tween 20 or polyethylene glycol followed by staining with 0.01% Coomassie Brilliant Blue. No destaining of

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Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.

Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.

Questions

  1. How should product B8647-1ea be used? Should the contents of the bottle be removed into a calibrated cylinder, water added to reach a final volume of 1 L, and then replaced all into the bottle?

    1 answer
    1. the bottle for B8647 contains 465ml of concentrate. One option is to pour out the contents into a calibrated volumetric flask and add sufficient water to make it 1000 ml. Once reconstituted to 1 liter, the contents of the flask could then be returned to the bottle for B8647. However, this process may seem labor-intensive. Alternatively, most users will likely choose to add 535 ml of water to the 1 liter bottle of concentrate and mix well.

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