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Merck

I3146

Sigma-Aldrich

ITS液体培养基补充剂(100x)

liquid, sterile-filtered, BioReagent, suitable for cell culture

别名:

Human ITS Liquid Media, Insulin-transferrin-sodium selenite

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About This Item

UNSPSC代码:
12352205
NACRES:
NA.75
价格与库存信息目前不能提供

无菌性

sterile-filtered

产品线

BioReagent

表单

liquid

技术

cell culture | mammalian: suitable
single cell analysis: suitable

杂质

endotoxin, tested

运输

ambient

储存温度

2-8°C

应用

胰岛素-转铁蛋白-硒添加剂(100×ITS液体培养基添加剂)作为培养基补充剂适用于:
  • α小鼠肝12细胞(AML-12)[1]
  • 大鼠胎儿睾丸外植体[2]
  • 人牙髓干细胞(DPSC)[3]

生化/生理作用

  • 胰岛素:一种多肽激素,可促进葡萄糖和氨基酸的摄取,这种特性可能与观察到的有丝分裂作用有关。[4]
  • 转铁蛋白:铁运输蛋白。
  • 硒:通常由血清提供的必需微量元素。[5]

组分

含有1.0 mg/ml人重组胰岛素,0.55 mg/ml 人转铁蛋白(基本上不含铁)以及0.5 μg/ml 100x浓度的亚硒酸钠。

其他说明

ITS是人重组胰岛素、人转铁蛋白和亚硒酸钠的一种混合物。它是一种通用的细胞补充剂设计用于含有丙酮酸钠的非复合型培养基(如MEM、RPMI-1640)及复合型培养基(如Ham?s F-12、DME/F-12、MEM)。

制备说明

使用不含酚红的Earle′平衡盐溶液(EBSS)制备。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Kelvin S C Cheung et al.
PloS one, 9(6), e98063-e98063 (2014-06-04)
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Translationally controlled tumor protein (TCTP), which is a protein characterized by its potent proliferation promoting activity, has been well studied in the area of growth and tumorigenesis. However, the specific role of TCTP in liver regeneration (LR) and its underlying

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Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.

Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.

Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.

Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.

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Questions

1–3 of 3 Questions  
  1. What should you use to dilute this to a 1X solution?

    1 answer
    1. This product can be dissolved in a given target cell culture medium, e.g., non-complex media (MEM, RPMI-1640), to a 1X solution.

      Helpful?

  2. What are the product numbers for the stand alone components in I3146?

    1 answer
    1. The product numbers for the stand-alone components in I3146 are as follows:
      Insulin – I2643 or I3536 (sterile)
      Apo-Transferrin – T2252
      Sodium Selenite – S5261
      EBBS – E3024

      Helpful?

  3. The ITS product #I3146 has been on back order for a while. Is there an alternative with similar components?

    1 answer
    1. Based on the Media Supplement Formulation, the closest alternative to product No. I3146 is the I2521 ITS+1 Liquid Media Supplement (100×), which also contains BSA and Linoleic acid.

      Helpful?

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